Recombinant mouse CHST5 protein

CHST5, also known as Carbohydrate sulfotransferase 5, is a Golgi-embedded enzyme that is found in T cells, B cells and intestinal epithelium. This sulfotransferase utilizes 3-phospho-5-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues of keratan. It also mediates sulfation of keratan in cornea. It acts on the non-reducing terminal GlcNAc of short and long carbohydrate substrates that have poly-N-acetyllactosamine structures. It may also have activity toward O-linked sugars of mucin-type acceptors. There is no CHST6 found in the mouse genome it is possible that mouse CHST5 plays a similar biological role to the human CHST6. Recombinant mouse CHST5, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.