Recombinant mouse CHST5 protein

CHST5, also known as Carbohydrate sulfotransferase 5, is a Golgi-embedded enzyme that is found in T cells, B cells and intestinal epithelium. This sulfotransferase utilizes 3-phospho-5-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues of keratan. It also mediates sulfation of keratan in cornea. It acts on the non-reducing terminal GlcNAc of short and long carbohydrate substrates that have poly-N-acetyllactosamine structures. It may also have activity toward O-linked sugars of mucin-type acceptors. There is no CHST6 found in the mouse genome it is possible that mouse CHST5 plays a similar biological role to the human CHST6. Recombinant mouse CHST5, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is > 10,000 pmol/min/ug, and is defined as the amount of enzyme that sulfate from PAPS to N-acetyl-D-glucosamine per minute at pH 7.5, at 37C.

Product Name Alternative

Chst5, Carbohydrate sulfotransferase 5, Galactose, N-acetylglucosamine, N-acetylglucosamine 6-O-sulfotransferase 4, GST4, Intestinal N-acetylglucosamine-6-O-sulfotransferase, I-GlcNAc6ST, Intestinal GlcNAc-6-sulfotransferase, mIGn6ST, N-acetylglucosamine 6-O-sulfotransferase 3, GlcNAc6ST-3, Gn6st-3, I-GlcNAc-6-ST, I-GlcN, GST-4. GST-

Expression System

Baculovirus

Antigen Species

Mouse

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

0.25 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS - PAGE

Bioactivity

Specific activity is > 10,000 pmol/min/ug, and is defined as the amount of enzyme that sulfate from PAPS to N-acetyl-D-glucosamine per minute at pH 7.5, at 37C.

Molecular Weight

42.9kDa (380aa)

Additionnal Information

, ATGP3995-10 µg, ATGP3995-20 µg, ATGP3995-50 µg, ATGP3995-100 µg, ATGP3995-250 µg, ATGP3995-500 µg, ATGP3995-1 mg, ATGP3995-10, ATGP3995-20, ATGP3995-50, ATGP3995-100, ATGP3995-250, ATGP3995-500, ATGP3995-1

References & Citations

Narentuya., et al, (2019) Sci Rep 9:4387.; ; Yasutaka Hayashida., et al, (2006) Proc Natl Acad Sci U S A 103:13333-13338.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol

Scientific Category

Immunology

NCBI Accession Number

NP_064334

Uniprot Accession Number

Q9QUP4

Species

Mouse

N Terminal Sequence

Ala

AA Sequence

SRQVP SSPAGLGERV HVLVLSSWRS GSSFVGQLFS QHPDVFYLME PAWHVWDTLS QGSAPALHMA VRDLIRSVFL CDMDVFDAYL PWRRNISDLF QWAVSRALCS PPVCEAFARG NISSEEVCKP LCATRPFGLA QEACSSYSHV VLKEVRFFNL QVLYPLLSDP ALNLRIVHLV RDPRAVLRSR EQTAKALARD NGIVLGTNGT WVEADPRLRV VNEVCRSHVR IAEAALHKPP PFLQDRYRLV RYEDLARDPL TVIRELYAFT GLGLTPQLQT WIHNITHGSG PGARREAFKT TSRDALSVSQ AWRHTLPFAK IRRVQELCGG ALQLLGYRSV HSELEQRDLS LDLLLPRGMD SFKWASSTEK QPES