What is the catalog number for Recombinant human FAP protein?

The catalog number for Recombinant human FAP protein is ATGP3988-100.

What sizes are available for Recombinant human FAP protein?

Recombinant human FAP protein is available in the following size(s): 100 µg.

Is Recombinant human FAP protein in stock?

Recombinant human FAP protein is currently made to order / not held in immediate stock. Contact us for lead time.

Recombinant human FAP protein

FAP, also known as Seprase, is a homodimeric integral membrane gelatinase belonging to the serine protease family. FAP includes dipeptidyl peptidase IV (DPPIV / CD26) and related type II transmembrane prolyl serine peptidases, which exert their mechanisms of action on the cell surface. And it's enzymatic activity is dependent on FAP association with DPPIV on the cell surface. The active site of FAP is localized in the extracellular part of the protein and contains a catalytic triad composed of Ser624 Asp702 His734 in humans and mice. It is catalytically active as a 170kD homodimer and has a dipeptidase and an endopeptidase activity. FAP expression is high in reactive stromal fibroblasts of epithelial cancers, granulation tissue of healing wounds, and malignant cells of bone and soft tissue sarcomas. FAP is thought to be involved in the control of fibroblast growth or epithelial-mesenchymal interactions during development, tissue repair, and epithelial carcinogenesis. ecombinant human FAP, fused to His-tag at C-terminus, was expressed in insert cell and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is > 5,000 pmol/min/ug, and is defined as the amount of enzyme that hydrolysis 1.0 pmole of Z-GP-AMC per minute at pH 7.5, at 37C.

Product Name Alternative

Fibroblast activation protein, DPPIV Protein, DPPIV, FAPA, Fapalpha Protein, SIMP Protein, Prolyl endopeptidase FAP, 170 kDa melanoma membrane-bound gelatinase, Dipeptidyl peptidase FAP, Fibroblast activation protein alpha, FAPalpha, Gelatine degradation protease FAP, Integral membrane serine protease, Post-proline cleaving enzyme, Serine integral membrane protease, SIMP, Surface-expressed protease, Seprase

Expression System

Baculovirus

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

0.25 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Bioactivity

Specific activity is > 5,000 pmol/min/ug, and is defined as the amount of enzyme that hydrolysis 1.0 pmole of Z-GP-AMC per minute at pH 7.5, at 37C.

Molecular Weight

86.1 kDa (744aa)

Additionnal Information

, ATGP3988-10 µg, ATGP3988-20 µg, ATGP3988-50 µg, ATGP3988-100 µg, ATGP3988-250 µg, ATGP3988-500 µg, ATGP3988-1 mg, ATGP3988-10, ATGP3988-20, ATGP3988-50, ATGP3988-100, ATGP3988-250, ATGP3988-500, ATGP3988-1

References & Citations

Scanlan, M.J. et al. (1994) Proc. Natl. Acad. Sci. USA 91:5657-5661.; ; Mori, Y. et al., 2004, Oncology. 67 (5-6) :411-419.; ; O'Brien, P. et al., 2008, Biochim Biophys Acta. 1784 (9) :1130-1145.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol

Scientific Category

Cancer, Enzymes & Proteases, Cell Cycle

NCBI Accession Number

NP_004451.2

Uniprot Accession Number

Q12884

Species

Human

N Terminal Sequence

Ala

AA Sequence

LRPSRVH NSEENTMRAL TLKDILNGTF SYKTFFPNWI SGQEYLHQSA DNNIVLYNIE TGQSYTILSN RTMKSVNASN YGLSPDRQFV YLESDYSKLW RYSYTATYYI YDLSNGEFVR GNELPRPIQY LCWSPVGSKL AYVYQNNIYL KQRPGDPPFQ ITFNGRENKI FNGIPDWVYE EEMLATKYAL WWSPNGKFLA YAEFNDTDIP VIAYSYYGDE QYPRTINIPY PKAGAKNPVV RIFIIDTTYP AYVGPQEVPV PAMIASSDYY FSWLTWVTDE RVCLQWLKRV QNVSVLSICD FREDWQTWDC PKTQEHIEES RTGWAGGFFV STPVFSYDAI SYYKIFSDKD GYKHIHYIKD TVENAIQITS GKWEAINIFR VTQDSLFYSS NEFEEYPGRR NIYRISIGSY PPSKKCVTCH LRKERCQYYT ASFSDYAKYY ALVCYGPGIP ISTLHDGRTD QEIKILEENK ELENALKNIQ LPKEEIKKLE VDEITLWYKM ILPPQFDRSK KYPLLIQVYG GPCSQSVRSV FAVNWISYLA SKEGMVIALV DGRGTAFQGD KLLYAVYRKL GVYEVEDQIT AVRKFIEMGF IDEKRIAIWG WSYGGYVSSL ALASGTGLFK CGIAVAPVSS WEYYASVYTE RFMGLPTKDD NLEHYKNSTV MARAEYFRNV DYLLIHGTAD DNVHFQNSAQ IAKALVNAQV DFQAMWYSDQ NHGLSGLSTN HLYTHMTHFL KQCFSLSD