Recombinant human FOLR2 protein
FOLR2, also known as Folate receptor beta, is a member of the folate receptor (FLOR) family. Members of this gene family have a high affinity for folate and folic acid analogs at neutral pH. And they mediate delivery of 5-methyltetrahydrofolate to the interior of cells. It was originally thought to exist only in placenta, but is also expressed in placenta, cells of the neutrophilic lineage, and some CD34+ hematopoietic progenitor cells. FOLR2 is also upregulated on macrophages and monocytes at chronic inflammatory sites including rheumatoid arthritis synovium and glioblastoma. Recombinant human FOLR2, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Folic Acid-BSA is coated at 10 µg/mL (100 ul/well) can bind Human FOLR2. The ED50 range ≤ 5 µg/mL.
Product Name Alternative
Expression System
HEK293
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE, Bioactivity
Concentration
0.25 mg/mL (determined by absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 90% by SDS-PAGE
Bioactivity
Measured by its binding ability in a functional ELISA with Folic Acid-BSA. The ED50 range ≤ 5 µg/mL.
Molecular Weight
25.1 kDa (215aa)
Additionnal Information
References & Citations
Ross, J.F. et al.,1994, Cancer 73:2432.; ; Joost W van der Heijden J.W. et al., 2009, Arthritis Rheum. 60:12-21.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Scientific Category
Signal Transduction, Metabolism
NCBI Accession Number
NP_000794.3
Uniprot Accession Number
P14207
Species
Human
N Terminal Sequence
Gln 22
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