Recombinant canine IL-5 protein
IL-5, also known as interleukin-5, is a member of the hematopoietin receptor superfamily and is comprised of a cytokine-specific alpha chain and the common beta chain that is shared among these cytokines for signaling. Through binding to the IL-5 receptor, IL-5 stimulates B cell growth and increases immunoglobulin secretion. It related hematopoietic cytokines that are important for allergic inflammation. It is a lineage-specific cytokine for eosinophilpoiesis and plays an important part in diseases associated with increased eosinophils, such as asthma. Recombinant canine IL-5, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques
Product Specifications
Specifications
Canine IL-5 in a cell proliferation assay using TF-1 human erythroleukemic cell. The ED50 range ≤ 15 ng/mL.
Product Name Alternative
Interleukin-5, Eosinophil differentiation factor, T-cell replacing factor, TRF, EDF
Expression System
Baculovirus
Antigen Species
Canine
Tag
His-Tag
Applications
SDS-PAGE, Bioactivity
Concentration
0.25 mg/mL (determined by Absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 90% by SDS - PAGE
Bioactivity
Measured in a cell proliferation assay using TF-1 human erythroleukemic cell. The ED50 range ≤ 15 ng/mL.
Molecular Weight
13.9kDa (119aa)
Additionnal Information
References & Citations
Martinez-Moczygemba M., et al. (2003) J Allergy Clin Immunol. 112:653-665.; ; Milburn MV., et al. (1993) Nature. 363:172-176.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Scientific Category
Immunology, Cancer
NCBI Accession Number
NP_001006951
Uniprot Accession Number
Q95J76
Species
Canine
N Terminal Sequence
Val 22
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