Recombinant canine IL-5 protein

IL-5, also known as interleukin-5, is a member of the hematopoietin receptor superfamily and is comprised of a cytokine-specific alpha chain and the common beta chain that is shared among these cytokines for signaling. Through binding to the IL-5 receptor, IL-5 stimulates B cell growth and increases immunoglobulin secretion. It related hematopoietic cytokines that are important for allergic inflammation. It is a lineage-specific cytokine for eosinophilpoiesis and plays an important part in diseases associated with increased eosinophils, such as asthma. Recombinant canine IL-5, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques

Product Specifications

Specifications

Canine IL-5 in a cell proliferation assay using TF-1 human erythroleukemic cell. The ED50 range ≤ 15 ng/mL.

Product Name Alternative

Interleukin-5, Eosinophil differentiation factor, T-cell replacing factor, TRF, EDF

Expression System

Baculovirus

Antigen Species

Canine

Tag

His-Tag

Applications

SDS-PAGE, Bioactivity

Concentration

0.25 mg/mL (determined by Absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS - PAGE

Bioactivity

Measured in a cell proliferation assay using TF-1 human erythroleukemic cell. The ED50 range ≤ 15 ng/mL.

Molecular Weight

13.9kDa (119aa)

Additionnal Information

B cell growth factor II, B-cell growth factor II, BCGF I, BCGF II, BCGFI, BCGF-I, BCGFII, BCGF-II, EDF, Eosinophil differentiation factor, IL 5, IL5, IL-5, Interleukin 5, Interleukin5, Interleukin-5, T cell replacing factor, T-cell replacing factor, TRF, ATGP3985-10 µg, ATGP3985-20 µg, ATGP3985-50 µg, ATGP3985-100 µg, ATGP3985-250 µg, ATGP3985-500 µg, ATGP3985-1 mg, ATGP3985-010, ATGP3985-020, ATGP3985-050, ATGP3985-100, ATGP3985-250, ATGP3985-500, ATGP3985-01M