Recombinant human pIgR protein

PIgR, also known as polymeric immunoglobulin receptor, is responsible for transcytosis of soluble dimeric IgAs and immune. This protein binds polymeric IgA and IgM at the basolateral surface of epithelial cells. The complex is then transported across the cell to be secreted at the apical surface. During this process, the cleavage occurs that separates the extracellular from the transmembrane segment. PIgR expression is under the strong regulation of cytokines, hormones, and pathogenic stimuli. The expression of pIgR is critically regulated by the pro-inflammatory cytokines, such as IL-1, IL-4, TNF-α, and IFN-γ. Recombinant human pIgR, fused to Histag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Human IgM is coated at 2 µg/mL (100 ul/well) can bind Human pIgR. The ED50 range ≤10 µg/mL.

Product Name Alternative

Polymeric immunoglobulin receptor, PIGR, PIgR, Poly-Ig receptor, Hepatocellular carcinoma-associated protein TB6, polymeric immunoglobulin receptor precursor

Expression System

Baculovirus

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Bioactivity

Concentration

1 mg/mL (determined by Absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS - PAGE

Bioactivity

Measured by its binding ability in a functional ELISA with Human IgM. The ED50 range ≤10 µg/mL.

Molecular Weight

68.9kDa (629aa)

Additionnal Information

, ATGP3978-10 µg, ATGP3978-20 µg, ATGP3978-50 µg, ATGP3978-100 µg, ATGP3978-250 µg, ATGP3978-500 µg, ATGP3978-1 mg, ATGP3978-10, ATGP3978-20, ATGP3978-50, ATGP3978-100, ATGP3978-250, ATGP3978-500, ATGP3978-1

References & Citations

Giffroy D, et al, (2001) Scand J Immunol. 53:56-64.; ; Kortum AN, et al, (2014) Immunogenetics. 66:267-279.; ; Yue X, et al, (2017) Hepatolgoy. 65:1948-1962.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Immunology

NCBI Accession Number

NP_002635

Uniprot Accession Number

P01833

Species

Human

N Terminal Sequence

Ala

AA Sequence

KSPIFGP EEVNSVEGNS VSITCYYPPT SVNRHTRKYW CRQGARGGCI TLISSEGYVS SKYAGRANLT NFPENGTFVV NIAQLSQDDS GRYKCGLGIN SRGLSFDVSL EVSQGPGLLN DTKVYTVDLG RTVTINCPFK TENAQKRKSL YKQIGLYPVL VIDSSGYVNP NYTGRIRLDI QGTGQLLFSV VINQLRLSDA GQYLCQAGDD SNSNKKNADL QVLKPEPELV YEDLRGSVTF HCALGPEVAN VAKFLCRQSS GENCDVVVNT LGKRAPAFEG RILLNPQDKD GSFSVVITGL RKEDAGRYLC GAHSDGQLQE GSPIQAWQLF VNEESTIPRS PTVVKGVAGG SVAVLCPYNR KESKSIKYWC LWEGAQNGRC PLLVDSEGWV KAQYEGRLSL LEEPGNGTFT VILNQLTSRD AGFYWCLTNG DTLWRTTVEI KIIEGEPNLK VPGNVTAVLG ETLKVPCHFP CKFSSYEKYW CKWNNTGCQA LPSQDEGPSK AFVNCDENSR LVSLTLNLVT RADEGWYWCG VKQGHFYGET AAVYVAVEER KAAGSRDVSL AKADAAPDEK VLDSGFREIE NKAIQDPRLF AEEKAVADTR DQADGSRASV DSGSSEEQGG SSR