Recombinant mouse sFRP-2/SFRP2 protein

SFRP-2, also known as Secreted apoptosis-related protein 1, is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. sFRP-2 can exert either positive or negative effects on Wnt signaling. At physiological concentrations, it also inhibits BMP-induced effects. It plays a variety of roles during tissue morphogenesis including inhibition of the planar cell polarity pathway and myoblast and osteoblast differentiation. It is also expressed in multiple myeloma and glioma in which it promotes tumorigenicity. Recombinant mouse sFRP-2, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

SFRP-2, sFRP2, Secreted frizzled-related protein 2, Secreted apoptosis-related protein 1, SDF-5, Sdf5, sdf, SARP-1, SARP1, Protein SDF5, FRP-2secreted apoptosis related protein 1, AI851596

Expression System

Baculovirus

Antigen Species

Mouse

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Molecular Weight

32.1kDa (280aa)

Additionnal Information

, ATGP3973-10 µg, ATGP3973-20 µg, ATGP3973-50 µg, ATGP3973-100 µg, ATGP3973-250 µg, ATGP3973-500 µg, ATGP3973-1 mg, ATGP3973-10, ATGP3973-20, ATGP3973-50, ATGP3973-100, ATGP3973-250, ATGP3973-500, ATGP3973-1

References & Citations

Alfaro, M.P. et al. (2010) J. Biol. Chem. 285:35645-35653; ; He, W. et al. (2010) Proc. Natl. Acad. Sci. 107:21110-21115;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 100 mM NaCl, 20% glycerol

Scientific Category

Signal Transduction

NCBI Accession Number

NP_033170.1

Uniprot Accession Number

P97299

Species

Mouse

N Terminal Sequence

Ala

AA Sequence

LFLFGQP DFSYKRSNCK PIPANLQLCH GIEYQNMRLP NLLGHETMKE VLEQAGAWIP LVMKQCHPDT KKFLCSLFAP VCLDDLDETI QPCHSLCVQV KDRCAPVMSA FGFPWPDMLE CDRFPQDNDL CIPLASSDHL LPATEEAPKV CEACKTKNED DNDIMETLCK NDFALKIKVK EITYINRDTK IILETKSKTI YKLNGVSERD LKKSVLWLKD SLQCTCEEMN DINAPYLVMG QKQGGELVIT SVKRWQKGQR EFKRISRSIR KLQC