Recombinant mouse sFRP-2/SFRP2 protein
SFRP-2, also known as Secreted apoptosis-related protein 1, is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. sFRP-2 can exert either positive or negative effects on Wnt signaling. At physiological concentrations, it also inhibits BMP-induced effects. It plays a variety of roles during tissue morphogenesis including inhibition of the planar cell polarity pathway and myoblast and osteoblast differentiation. It is also expressed in multiple myeloma and glioma in which it promotes tumorigenicity. Recombinant mouse sFRP-2, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Expression System
Baculovirus
Antigen Species
Mouse
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 90% by SDS-PAGE
Molecular Weight
32.1kDa (280aa)
Additionnal Information
References & Citations
Alfaro, M.P. et al. (2010) J. Biol. Chem. 285:35645-35653; ; He, W. et al. (2010) Proc. Natl. Acad. Sci. 107:21110-21115;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 100 mM NaCl, 20% glycerol
Scientific Category
Signal Transduction
NCBI Accession Number
NP_033170.1
Uniprot Accession Number
P97299
Species
Mouse
N Terminal Sequence
Ala
AA Sequence
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