What is the catalog number for Recombinant human Thrombopoietin protein?

The catalog number for Recombinant human Thrombopoietin protein is ATGP3970-050.

What sizes are available for Recombinant human Thrombopoietin protein?

Recombinant human Thrombopoietin protein is available in the following size(s): 50 µg.

Is Recombinant human Thrombopoietin protein in stock?

Recombinant human Thrombopoietin protein is currently made to order / not held in immediate stock. Contact us for lead time.

Recombinant human Thrombopoietin protein

Thrombopoietin, also known as megakaryocyte growth and development factor (MGDF), is a member of EPO/TPO family. It is produced in the liver by both parenchymal cells and sinusoidal endothelial cells, as well as in the kidney by proximal convoluted tubule cells. It acts as a key regulator of megakaryocytopoiesis and thrombopoiesis that leads to platelet production. Also, this protein is the ligand for MLP/C_MPL, the product of myeloproliferative leukemia virus oncogene. Thrombopoietin, like EPO, plays a role in brain development. It promotes apoptosis of newly generated neurons, an effect counteracted by EPO and neurotrophins. Recombinant human Thrombopoietin fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Human Thrombopoietin stimulates cell proliferation of the MO7e human megakaryocytic leukemic cells. The ED50 range ≤10 ng/mL.

Product Name Alternative

THPO, MGDF, MKCSF, ML, MPLLG, THCYT1, TPO, thrombopoietin isoform 1 precursor, C-mpl ligand, Megakaryocyte colony-stimulating factor, Megakaryocyte growth and development factor, Myeloproliferative leukemia virus oncogene ligand, ATGP3057

Expression System

HEK293

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Bioactivity

Concentration

0.25 mg/mL (determined by Absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Bioactivity

Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED50 range ≤10 ng/mL.

Molecular Weight

36.8kDa (343aa)

Additionnal Information

, ATGP3970-10 µg, ATGP3970-20 µg, ATGP3970-50 µg, ATGP3970-100 µg, ATGP3970-250 µg, ATGP3970-500 µg, ATGP3970-1 mg, ATGP3970-10, ATGP3970-20, ATGP3970-50, ATGP3970-100, ATGP3970-250, ATGP3970-500, ATGP3970-1

References & Citations

Fishley B, et al, (2004) Growth Factors. 22:151-155.; ; Chou FS, et al, (2011) J Cell Biochem. 112:1491-1498.; ; Kong Z, et al, (2017) Blood. 130:1097-1103.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Cardiovascular

NCBI Accession Number

NP_000451.1

Uniprot Accession Number

P40225

Species

Human

N Terminal Sequence

Asp

AA Sequence

SPAPP ACDLRVLSKL LRDSHVLHSR LSQCPEVHPL PTPVLLPAVD FSLGEWKTQM EETKAQDILG AVTLLLEGVM AARGQLGPTC LSSLLGQLSG QVRLLLGALQ SLLGTQLPPQ GRTTAHKDPN AIFLSFQHLL RGKVRFLMLV GGSTLCVRRA PPTTAVPSRT SLVLTLNELP NRTSGLLETN FTASARTTGS GLLKWQQGFR AKIPGLLNQT SRSLDQIPGY LNRIHELLNG TRGLFPGPSR RTLGAPDISS GTSDTGSLPP NLQPGYSPSP THPPTGQYTL FPLPPTLPTP VVQLHPLLPD PSAPTPTPTS PLLNTSYTHS QNLSQEG