Recombinant mouse LIFR alpha/LIFR Protein
LIFR alpha, also known as CD118, is a member of the Interleukin-6 receptor family. Members of this family mediate the biological effects of Cardiotrophin-1, CLC, CNTF, IL-6, IL-11, IL-27, and Oncostatin M. The leukemia inhibitory factor is a polyfunctional cytokine that affects the differentiation, survival, and proliferation of a wide variety of cells in the adult and the embryo. LIFR alpha has also been identified as a breast cancer metastasis suppressor that functions through the HIPPO-YAP pathway. LIFR alpha is down regulated in a number of breast carcinomas and may serve a prognostic tool. Recombinant mouse LIFR alpha, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Mouse LIFR alpha inhibits human LIF (Cat# ATGP3533) induced cell proliferation in the TF-1 human erythroleukemic cells. The ED50 range is ≤ 1 µg/mL.
Product Name Alternative
Leukemia inhibitory factor receptor, LIF receptor, LIF-R, LIF receptor subunit alpha, D-factor, CD118
Expression System
Baculovirus
Antigen Species
Mouse
Tag
His-Tag
Applications
SDS-PAGE, Bioactivity
Concentration
0.25 mg/mL (determined by Absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 90% by SDS-PAGE
Bioactivity
Measured by its ability to inhibit proliferation using TF-1 human erythroleukemic cells. The ED50 range ≤ 1 µg/mL with Mouse LIF.
Molecular Weight
89.6kDa (794aa)
Additionnal Information
References & Citations
Gearing DP., et al (1991) The EMBO Journal. 10: 2839–2848.; ; Layton MJ., et al. (1992) Proc. Natl. Acad. Sci. 89:8616.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Scientific Category
Immunology
NCBI Accession Number
NP_038612
Uniprot Accession Number
P42703
Species
Mouse
N Terminal Sequence
Leu
AA Sequence
Frequently Asked Questions
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