Recombinant canine IL-1 beta/IL1B protein

Interleukin-1 (IL-1) has been considered a potentially important inflammatory mediator. It is composed of two distinct proteins, called as IL-1alpha and IL-1beta. IL-1beta is a proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL-1alpha and IL-1beta bind to the same receptor and they are structurally related polypeptides that share approximately 20% amino acid. Recombinant canine IL-1beta was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Canine IL-1B stimulates cell proliferation of the D10.G4.1 mouse helper T cells. The ED50 range range ≤ 5pg/ml.

Product Name Alternative

Interleukin-1 beta, IL-1 beta

Expression System

E.coli

Antigen Species

Canine

Tag

Non-Tagged

Applications

SDS-PAGE, Bioactivity

Concentration

1 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Bioactivity

Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED50 range ≤ 5pg/ml.

Molecular Weight

17.5 kDa (153aa) confirmed by MALDI-TOF

Additionnal Information

Beta proprotein, Catabolin, IL 1, IL 1 b, IL 1b, IL 1beta, IL 1 beta, IL1, IL-1, IL1 b, IL1 beta, IL1b, IL-1b, IL1-beta, IL-1beta, IL-1-beta, IL1F2, Interleukin 1, Interleukin 1 b, Interleukin 1 beta, Interleukin 1b, Interleukin 1beta, ATGP3956-10 µg, ATGP3956-20 µg, ATGP3956-50 µg, ATGP3956-100 µg, ATGP3956-250 µg, ATGP3956-500 µg, ATGP3956-1 mg, ATGP3956-010, ATGP3956-020, ATGP3956-050, ATGP3956-100, ATGP3956-250, ATGP3956-500, ATGP3956-01M

References & Citations

Allan SM., et al. (2005), Nat. Rev. Immunol. 5:629; ; Curr Pharm Des. (2017), 23 (32) :4893-4905.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4)

Scientific Category

Hormones & Cytokines

NCBI Accession Number

NP_001033060.1

Uniprot Accession Number

Q2VCE2

Species

Canine

AA Sequence

MAAMQSVDCK LQDISHKYLV LSNSYELRAL HLNGENVNKQ VVFHMSFVHG DESNNKIPVV LGIKQKNLYL SCVMKDGKPT LQLEKVDPKV YPKRKMEKRF VFNKIEIKNT VEFESSQYPN WYISTSQVEG MPVFLGNTRG GQDITDFTME FSS