Recombinant canine IL-1 beta/IL1B protein
Interleukin-1 (IL-1) has been considered a potentially important inflammatory mediator. It is composed of two distinct proteins, called as IL-1alpha and IL-1beta. IL-1beta is a proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL-1alpha and IL-1beta bind to the same receptor and they are structurally related polypeptides that share approximately 20% amino acid. Recombinant canine IL-1beta was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Canine IL-1B stimulates cell proliferation of the D10.G4.1 mouse helper T cells. The ED50 range range ≤ 5pg/ml.
Product Name Alternative
Interleukin-1 beta, IL-1 beta
Expression System
E.coli
Antigen Species
Canine
Tag
Non-Tagged
Applications
SDS-PAGE, Bioactivity
Concentration
1 mg/mL (determined by absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 95% by SDS-PAGE
Bioactivity
Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED50 range ≤ 5pg/ml.
Molecular Weight
17.5 kDa (153aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Allan SM., et al. (2005), Nat. Rev. Immunol. 5:629; ; Curr Pharm Des. (2017), 23 (32) :4893-4905.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4)
Scientific Category
Hormones & Cytokines
NCBI Accession Number
NP_001033060.1
Uniprot Accession Number
Q2VCE2
Species
Canine
AA Sequence
Frequently Asked Questions
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