Recombinant mouse Kell protein
Kell, also known as Kell blood group glycoprotein homolog, is a member of the zinc endopeptidase of the neprilysin (NEP) family. It is an enzyme whose principal known function is the production of a potent bioactive peptide, ET-3. It also includes endothelin converting enzymes (ECE-1 and ECE-2), PEX, XCE, DINE and several NEP-like proteins. It is linked through a single disulfide bond to XK, a putative membrane transporter. The two proteins constitute the Kell blood group antigens. The Kell antigen system (also known as Kell–Cellano system) is one of the most clinically important blood group systems. Recombinant mouse Kell, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Expression System
Baculovirus
Antigen Species
Mouse
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 90% by SDS-PAGE
Molecular Weight
76.3kDa (674aa)
Additionnal Information
References & Citations
Lee, S. et al. (1999) Blood 94:1440-1450.; ; Lee, S. et al. (2000) Transfus. Med. Rev. 14:93-103.; ; Turner, A.J. et al. (2001) BioEssays. 23:261-269.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Scientific Category
Cardiovascular
NCBI Accession Number
NP_115929
Uniprot Accession Number
Q9EQF2
Species
Mouse
N Terminal Sequence
Ala49
AA Sequence
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