Recombinant human DLL4 protein
DLL4, also known as Delta-Like protein 4, is a membrane protein belonging to the Delta/Serrate/Lag2 (DSL) family of Notch ligands. It is predicted to encode a membrane-bound ligand, characterized by an extracellular region containing several EGF-like domains and a DSL domain required for receptor binding. DLL4 is expressed highly and selectively within the arterial endothelium and has been shown to function as a ligand for Notch 1 and Notch 4. The Notch signaling pathway is fundamental to proper cardiovascular development and is now recognized as an important player in tumor angiogenesis. Two key Notch ligands have been implicated in tumor angiogenesis, Delta-like 4 and Jagged1. Recombinant human DLL4, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Delta-like protein 4, Drosophila Delta homolog 4, Delta4, delta like canonical Notch ligand 4, AOS6, hdelta2
Expression System
HEK293
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 95% by SDS-PAGE
Molecular Weight
55.1kDa (504aa)
Additionnal Information
References & Citations
J R Shutter., et al, (2000) Genes Dev. 14:1313-1318.; ; J Dufraine., et al, (2008) Oncogene. 27:5132-5137.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Scientific Category
Neuroscience
NCBI Accession Number
NP_061947
Uniprot Accession Number
Q9NR61
Species
Human
N Terminal Sequence
Ser
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