Recombinant mouse Prostatic Acid Phosphatase/ACPP protein

ACPP, also known as Prostatic Acid Phosphatase, is a member of histidine acid phosphatase family. Prostatic Acid Phosphatase/ACPP catalyzes the hydrolysis of a variety of phosphate monoesters, including phosphorylated proteins. The activity optimum of Prostatic Acid Phosphatase/ACPP is in the pH range of 4 - 6, and the activity is inhibited by L (+) -tartrate. It has also lipid phosphatase activity and inactivates lysophosphatidic acid in seminal plasma. Recombinant mouse Prostatic Acid Phosphatase/ACPP protein, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is >80,000 unit/mg, and is defined as the amount of enzyme that hydrolyze 1.0 nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 5.0 at 37C.

Product Name Alternative

Acid phosphatase, prostate, ACP3, ACP-3, ACPP, EC 3.1.3.2, PAP, Prostatic Acid Phosphatase, prostatic acid phosphatase, 5-nucleotidase, 5'-NT, Acid phosphatase 3, Ecto-5'-nucleotidase, Fluoride-resistant acid phosphatase, FRAP, Thiamine monophosphatase, TMPase, A030005E02Rik, Lap, PAP, Ppal

Expression System

Baculovirus

Antigen Species

Mouse

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

0.5 mg/mL (determined by Absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Bioactivity

Specific activity is >80,000 unit/mg, and is defined as the amount of enzyme that hydrolyze 1.0 nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 5.0 at 37C.

Molecular Weight

41.3kDa (356aa)

Additionnal Information

, ATGP3953-10 µg, ATGP3953-20 µg, ATGP3953-50 µg, ATGP3953-100 µg, ATGP3953-250 µg, ATGP3953-500 µg, ATGP3953-1 mg, ATGP3953-10, ATGP3953-20, ATGP3953-50, ATGP3953-100, ATGP3953-250, ATGP3953-500, ATGP3953-1