Recombinant Rat UNC5H2/UNC5B protein

UNC5B/UNC5B, also known as Netrin receptor UNC5B, is one of four related receptors that comprise the UNC5 family and serve as receptors for the axon guidance molecule Netrin-1. Outside of the nervous system, UNC5B facilitates Netrin-1-mediated inhibition of capillary branching and leukocyte migration, as well as Netrin-1-mediated promotion of osteoclast differentiation. Also this protein activates DAPK1 which mediates apoptosis. It is believed to play a role in tumorgenesis. Additionally, It is thought to play a critical role in the development of atherosclerosis and the survival of renal tubular epithelial cells during acute kidney injury. Recombinant Rat UNC5B/UNC5B, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Unc-5 netrin receptor B, Unc5h2, unc-5 homolog 2, Protein unc-5 homolog B, netrin receptor UNC5B precursor, netrin receptor UNC5B, protein unc-5 homolog 2, unc5b

Expression System

HEK293

Antigen Species

Rat

Tag

HIgG-His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Molecular Weight

66.0kDa (590aa)

Additionnal Information

, ATGP3950-10 µg, ATGP3950-20 µg, ATGP3950-50 µg, ATGP3950-100 µg, ATGP3950-250 µg, ATGP3950-500 µg, ATGP3950-1 mg, ATGP3950-10, ATGP3950-20, ATGP3950-50, ATGP3950-100, ATGP3950-250, ATGP3950-500, ATGP3950-1

References & Citations

Bhat SA, et al, (2019) J Cell Commun Signal. 13:121-127.; ; Mediero A, et al, (2016) FASEB J. 30:3835-3844.; ; Liu J, et al, (2013) Tumour Biol. 34:2099-2108.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Signal Transduction

NCBI Accession Number

NP_071543

Uniprot Accession Number

O08722

Species

Rat

N Terminal Sequence

Asp

AA Sequence

GIDSGG QALPDSFPSA PAEQLPHFLL EPEDAYIVKN KPVELHCRAF PATQIYFKCN GEWVSQKGHV TQESLDEATG LRIREVQIEV SRQQVEELFG LEDYWCQCVA WSSSGTTKSR RAYIRIAYLR KNFDQEPLAK EVPLDHEVLL QCRPPEGVPV AEVEWLKNED VIDPAQDTNF LLTIDHNLII RQARLSDTAN YTCVAKNIVA KRRSTTATVI VYVNGGWSSW AEWSPCSNRC GRGWQKRTRT CTNPAPLNGG AFCEGQACQK TACTTVCPVD GAWTEWSKWS ACSTECAHWR SRECMAPPPQ NGGRDCSGTL LDSKNCTDGL CVLNQRTLND PKSRPLEPSG D