Recombinant human TIE2/TEK protein

TEK, also known as angiopoietin-1 receptor isoform 1, is an endothelial cell-specific receptor tyrosine kinase subfamily (RTK) . This protein acts as cell-surface receptor and regulates angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton. It has anti-inflammatory effects by preventing the leakage of proinflammatory plasma proteins and leukocytes from blood vessels and is required for normal angiogenesis and heart development during embryogenesis. Recombinant human TEK protein, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Human Angiopoietin-2 (CAT# ATGP3853) is coated at 5 µg/mL (100 ul/well) can bind Human TIE-2. The ED50 range ≤1 µg/mL.

Product Name Alternative

TEK, TEK receptor tyrosine kinase, VMCM, venous malformations, multiple cutaneous and mucosal, TEK tyrosine kinase, endothelial, TIE2, TIE-2, VMCM1, CD202b, angiopoietin-1 receptor, Hyk, AA517024, STK1, p140 TEK

Expression System

Baculovirus

Antigen Species

Human

Tag

HIgG-His-Tag

Applications

SDS-PAGE, Bioactivity

Concentration

0.5 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Bioactivity

Measured by its binding ability in a functional ELISA with Human Angiopoietin-2 (CAT# ATGP3853) . The ED50 range ≤1 µg/mL.

Molecular Weight

107.9 kDa (965aa)

Additionnal Information

Tie2, Angiopoietin-1 receptor isoform 1, TEK, CD202B, GLC3E, TIE-2, TIE2, VMCM, VMCM1, ATGP3947-10 µg, ATGP3947-20 µg, ATGP3947-50 µg, ATGP3947-100 µg, ATGP3947-250 µg, ATGP3947-500 µg, ATGP3947-1 mg, ATGP3947-010, ATGP3947-020, ATGP3947-050, ATGP3947-100, ATGP3947-250, ATGP3947-500, ATGP3947-01M, ATGP3762