Recombinant human TIE2/TEK protein
TEK, also known as angiopoietin-1 receptor isoform 1, is an endothelial cell-specific receptor tyrosine kinase subfamily (RTK) . This protein acts as cell-surface receptor and regulates angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton. It has anti-inflammatory effects by preventing the leakage of proinflammatory plasma proteins and leukocytes from blood vessels and is required for normal angiogenesis and heart development during embryogenesis. Recombinant human TEK protein, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Human Angiopoietin-2 (CAT# ATGP3853) is coated at 5 µg/mL (100 ul/well) can bind Human TIE-2. The ED50 range ≤1 µg/mL.
Product Name Alternative
Expression System
Baculovirus
Antigen Species
Human
Tag
HIgG-His-Tag
Applications
SDS-PAGE, Bioactivity
Concentration
0.5 mg/mL (determined by Bradford assay)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 90% by SDS-PAGE
Bioactivity
Measured by its binding ability in a functional ELISA with Human Angiopoietin-2 (CAT# ATGP3853) . The ED50 range ≤1 µg/mL.
Molecular Weight
107.9 kDa (965aa)
Additionnal Information
References & Citations
Kitajima D., et al, (2016) J Cancer. 7:600-607.; ; Kelly-Goss MR., et al, (2017) Sci Rep. 7:9049.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Scientific Category
Signal Transduction
NCBI Accession Number
NP_000450
Uniprot Accession Number
Q02763
Species
Human
N Terminal Sequence
Ala23
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