Recombinant mouse SPARC protein
SPARC, also known as osteonectin and BM40, is a secreted matricellular glycoprotein that belongs to a group of functionally related glycoproteins that includes tenascins C and X, thrombospondins 1 and 2, and osteopontin. It is a major bone-related protein that is also present in nonmineralized tissues and in platelets and is matricellular molecule regulating interactions between cells and their surrounding extracellular matrix (ECM) . This protein is produced by fibroblasts, capillary endothelial cells, platelets and macrophages, especially in areas of tissue morphogenesis and remodeling. It shows context-specific effects, but generally inhibits adhesion, spreading and proliferation, and promotes collagen matrix formation. It is abundantly expressed in bone, where it promotes osteoblast differentiation and inhibits adipogenesis. Recombinant mouse SPARC, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Basement-membrane protein 40, BM-40, Osteonectin, ON, Secreted protein acidic and rich in cysteine, Sparc
Expression System
Baculovirus
Antigen Species
Mouse
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Absorbance at 280 nm)
Endotoxin
<1 EU per 1ug of protein (determined by LAL method)
Purity
> 95% by SDS-PAGE
Molecular Weight
33.3kDa (291aa)
Additionnal Information
References & Citations
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Scientific Category
Cell Cycle
NCBI Accession Number
NP_033268
Uniprot Accession Number
P07214
Species
Mouse
N Terminal Sequence
Ala
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