Recombinant Human ACE Protein
ACE, also known as peptidyl-dipetidase A, is a zinc metallopeptidase important for blood pressure control and water and salt metabolism. it converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. It's able to inactivate bradykinin, a potent vasodilator. It has also a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety. Recombinant human ACE/CD143, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Specific activity is > 1,000 pmol/min/mg, and is defined as the amount of enzyme that cleaves 1pmol of Mca-RPPGFSAFK (Dnp) -OH per minute at 25C.
Product Name Alternative
Angiotensin-coverting enzyme, Dipeptidyl carboxypeptidase I, Kininase II, ACE1, CD143, DCP, DCP1
Expression System
Baculovirus
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE, Enzyme Activity
Concentration
0.25 mg/mL (determined by Absorbance at 280 nm)
Endotoxin
<1 EU per 1ug of protein (determined by LAL method)
Purity
> 90% by SDS-PAGE
Bioactivity
Specific activity is > 1,000 pmol/min/mg, and is defined as the amount of enzyme that cleaves 1pmol of Mca-RPPGFSAFK (Dnp) -OH per minute at 25C.
Molecular Weight
142kDa (1235aa)
Additionnal Information
References & Citations
Amati E., et al, (2018) Stem Cell Res Ther. 9:10.; ; Metzger R., et al, (2000) Atherosclerosis. 150:21-31.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Scientific Category
Cardiovascular
NCBI Accession Number
NP_000780
Uniprot Accession Number
P12821
Species
Human
N Terminal Sequence
Leu 30
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