What is the catalog number for Recombinant human SHP-2/PTPN11 protein?

The catalog number for Recombinant human SHP-2/PTPN11 protein is ATGP3943-250.

What sizes are available for Recombinant human SHP-2/PTPN11 protein?

Recombinant human SHP-2/PTPN11 protein is available in the following size(s): 250 µg.

Is Recombinant human SHP-2/PTPN11 protein in stock?

Recombinant human SHP-2/PTPN11 protein is currently made to order / not held in immediate stock. Contact us for lead time.

Recombinant human SHP-2/PTPN11 protein

SHP-2, also known as, tyrosine-protein phospatase non-receptor type 11 (PTPN11), is a member of the protein tyrosine phosphatase (PTP) family containing two Src homology 2 domains. This protein dephosphorylates tyrosine residues in proteins. It plays a stimulatory role in activation of the Erk/MAP kinase pathway by receptor tyrosine kinase signaling. Mutations in this protein are a cause of Noonan syndrome as well as acute myeloid leukemia. Recombinant human SHP-2, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is > 400 unit/mg, and is defined as the amount of enzyme that hydrolyze 1.0 nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 7.5 at 37C.

Product Name Alternative

Protein tyrosine phosphatase non-receptor type 11, NS1, Noonan syndrome 1, SH2 domain-containing protein tyrosine phosphatase 2, SHP-2, Protein-tyrosine phosphatase 1D, PTP-1D, Protein-tyrosine phosphatase 2C, PTP-2C, PTP2C, SHP2, BPTP3, SH-PTP2

Expression System

Baculovirus

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

1 mg/mL (determined by Absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Bioactivity

Specific activity is > 400 unit/mg, and is defined as the amount of enzyme that hydrolyze 1.0 nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 7.5 at 37C.

Molecular Weight

69.1kDa (602aa)

Additionnal Information

, ATGP3943-10 µg, ATGP3943-20 µg, ATGP3943-50 µg, ATGP3943-100 µg, ATGP3943-250 µg, ATGP3943-500 µg, ATGP3943-1 mg, ATGP3943-10, ATGP3943-20, ATGP3943-50, ATGP3943-100, ATGP3943-250, ATGP3943-500, ATGP3943-1

References & Citations

Giorgia R, et al, (2018) Cell Rep. 23:39-49. ; ; Chitranshi N., et al, (2017) Neuroscience. 364:175-189. ; ; Batth TS, et al, (2018) Cell Rep. 22:2784-2796. ;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol.

Scientific Category

Signal Transduction, Enzymes & Proteases, Epigenetics and Nuclear Signaling

NCBI Accession Number

NP_002825

Uniprot Accession Number

Q06124

Species

Human

N Terminal Sequence

Ala

AA Sequence

MTSRRWF HPNITGVEAE NLLLTRGVDG SFLARPSKSN PGDFTLSVRR NGAVTHIKIQ NTGDYYDLYG GEKFATLAEL VQYYMEHHGQ LKEKNGDVIE LKYPLNCADP TSERWFHGHL SGKEAEKLLT EKGKHGSFLV RESQSHPGDF VLSVRTGDDK GESNDGKSKV THVMIRCQEL KYDVGGGERF DSLTDLVEHY KKNPMVETLG TVLQLKQPLN TTRINAAEIE SRVRELSKLA ETTDKVKQGF WEEFETLQQQ ECKLLYSRKE GQRQENKNKN RYKNILPFDH TRVVLHDGDP NEPVSDYINA NIIMPEFETK CNNSKPKKSY IATQGCLQNT VNDFWRMVFQ ENSRVIVMTT KEVERGKSKC VKYWPDEYAL KEYGVMRVRN VKESAAHDYT LRELKLSKVG QGNTERTVWQ YHFRTWPDHG VPSDPGGVLD FLEEVHHKQE SIMDAGPVVV HCSAGIGRTG TFIVIDILID IIREKGVDCD IDVPKTIQMV RSQRSGMVQT EAQYRFIYMA VQHYIETLQR RIEEEQKSKR KGHEYTNIKY SLADQTSGDQ SPLPPCTPTP PCAEMREDSA RVYENVGLMQ QQKSFR