Recombinant human GDNF protein
GDNF, also known as Glial cell-derived neurotrophic factor, is a member of the GDNF family of ligands (GFL) . This Protein has been found that GFLs play a role in a number of biological processes including cell survival, neurite outgrowth, cell differentiation and cell migration. It promotes the survival of many types of neurons and signals through GFRa receptors, particularly GFRa1. These neuronal populations die in the course of Parkinson's disease and amyotrophic lateral sclerosis (ALS) . GDNF also regulates kidney development and spermatogenesis, and has a powerful and rapid negative (ameliorating) effect on alcohol consumption. Recombinant human GDNF, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Human GDNF is coated at 0.5 µg/mL (100 ul/well) can bind Human GFR alpha-1 (CAT# ATGP4154) in a Functional ELISA assay.
Product Name Alternative
Glial cell line-derived neurotrophic factor, hGDNF, Astrocyte-derived trophic factor, ATF, ATF1, ATF2, HFB1-GDNF, HSCR3
Expression System
Baculovirus
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE, Bioactivity
Concentration
0.25 mg/mL (determined by Absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 90% by SDS-PAGE
Bioactivity
Measured by its binding ability in a functional ELISA with Human GFR alpha-1 (CAT# ATGP4154) .
Molecular Weight
12.8kDa (113aa)
Additionnal Information
References & Citations
Lin LF., et al, (1993) Science. 260:1130–1132.; ; Carnicella S., et al, (2008) United States of America. 105: 8114–8119.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol.
Scientific Category
Immunology
NCBI Accession Number
NP_000505.1
Uniprot Accession Number
P39905
Species
Human
N Terminal Sequence
Ala
AA Sequence
RGQRGK NRGCVLTAIH LNVTDLGLGY ETKEELIFRY CSGSCDAAET TYDKILKNLS RNRRLVSDKV GQACCRPIAF DDDLSFLDDN LVYHILRKHS AKRCGCI
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