Recombinant human BMP-2 protein
BMP-2, also known as Bone morphogenetic protein 2, is multi-functional growth factor that belongs to the transforming growth factor beta (TGF beta) superfamily. It plays an important role in embryonic dorsal-ventral patterning, organogenesis, limb bud formation, and bone formation and regeneration. BMP-2 also promotes the maintenance and repair of colonic epithelium, suppresses neuronal dopamine synthesis and release, induces apoptosis in medulloblastoma cells, and is required for cardiac contractility. Recombinant human BMP2 was expressed in E. coli and purified by conventional chromatography techniques.
Product Specifications
Specifications
Human BMP-2 stimulates alkaline phosphatase in the ATDC5 mouse chondrogenic cells. The ED50 range ≤ 100 ng/mL.
Product Name Alternative
Bone morphogenetic protein 2, BMP-2A
Expression System
E.coli
Antigen Species
Human
Tag
Non-Tagged
Applications
SDS-PAGE, Bioactivity
Concentration
1 mg/mL (determined by Bradford assay)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 90% by SDS-PAGE
Bioactivity
Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED50 for this effect is ≤ 100 ng/mL.
Molecular Weight
13 kDa (115aa)
Additionnal Information
References & Citations
Chen D., et al. (2004) . Growth factors. 22 (4) :233-41; ; Schliephake H., et al. (2005) . Clin Oral Implants Res. 17 (6) :666-72
Other References
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 10 mM Sodium acetate (pH 3.5)
Scientific Category
Signal Transduction
NCBI Accession Number
NP_001191
Uniprot Accession Number
P12643
Species
Human
AA Sequence
MQAKHKQRKR LKSSCKRHPL YVDFSDVGWN DWIVAPPGYH AFYCHGECPF PLADHLNSTN HAIVQTLVNS VNSKIPKACC VPTELSAISM LYLDENEKVV LKNYQDMVVE GCGCR
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