Recombinant human IL-1Ra/IL1RN protein

IL-1RA/IL-1F3, also known as interleukin-1 receptor antagonist, is a glycosylated protein and a powerful anti-inflammatory agent. IL‐1RA is a pan‐physiological inhibitor of IL‐1 that prevents the activation of IL‐1 receptors by inhibiting IL‐1 binding to its receptors. It can inhibit angiogenesis by blocking the activation of the IL‐1α/PI3K/NF‐κB pathway in IL‐1‐producing human colon cancer cells. Also, Several autoimmune and inflammatory diseases have been associated with an allelic polymorphism of the IL-1RA. Recombinant human IL-1RA was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Human IL-1ra/IL-1F3 stimulates IFN-g secretion of the NK-92 human natural killer cells. The ED50 range ≤10 ng/mL.

Product Name Alternative

Interleukin-1 receptor antagonist protein, IL-1RN, IL-1ra, IRAP, ICIL-1RA, IL1 inhibitor, IL1F3, IL1RA

Expression System

E.coli

Antigen Species

Human

Tag

Non-Tagged

Applications

SDS-PAGE, Bioactivity

Concentration

1 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS - PAGE

Bioactivity

The activity is determined by the IFN-g ELISA in a using NK-92 human natural killer cells. The ED50 range ≤10 ng/mL.

Molecular Weight

17.2 kDa (153aa), confirmed by MALDI-TOF

Additionnal Information

DIRA, IL1F3, IL-1F3, IL-1ra3, F630041P17Rik, ICIL-1RA, Intracellular IL 1 receptor antagonist type II, Intracellular interleukin 1 receptor antagonist (icIL 1ra), MGC10430, MVCD4, Type II interleukin 1 receptor antagonist, ATGP3934-10 µg, ATGP3934-20 µg, ATGP3934-50 µg, ATGP3934-100 µg, ATGP3934-250 µg, ATGP3934-500 µg, ATGP3934-1 mg, ATGP3934-10, ATGP3934-20, ATGP3934-50, ATGP3934-100, ATGP3934-250, ATGP3934-500, ATGP3934-1

References & Citations

Capper., et al. (2004) Clin. Exp. Immunol. 138:348; ; Roberge CJ., et al. (1994) Journal of Immunology. 152: 5485-94; ; Carter DB., et al. (1990) Nature. 344: 633-7

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid. In Phosphate-Buffered Saline (pH7.4)

Scientific Category

Hormones & Cytokines

NCBI Accession Number

NP_776214

Uniprot Accession Number

P18510

Species

Human

AA Sequence

MRPSGRKSSK MQAFRIWDVN QKTFYLRNNQ LVAGYLQGPN VNLEEKIDVV PIEPHALFLG IHGGKMCLSC VKSGDETRLQ LEAVNITDLS ENRKQDKRFA FIRSDSGPTT SFESAACPGW FLCTAMEADQ PVSLTNMPDE GVMVTKFYFQ EDE