Recombinant human ST6GALNAC5 protein

ST6GALNAC5, also known as Alpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase 5, is a member of the glycosyltransferase 29 family. This protein is a sialyltransferase involved in the biosynthesis of ganglioside GD1a from GM1b. It is involved in the pathway protein glycosylation, which is part of Protein modification. It’s expression is restricted to the brain normally. It has been identified as a key player in the metastasis of breast cancer cells to the brain by potentially enabling the cancer cells to cross the blood-brain barrier. Recombinant human ST6GALNAC5, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Alpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase 5, GD1 alpha synthase, GalNAc alpha-2,6-sialyltransferase V, ST6GalNAc V, ST6GalNAcV, Sialyltransferase 7E, SIAT7-E, SIAT7E

Expression System

Baculovirus

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Molecular Weight

36.4 kDa (316aa)

Additionnal Information

, ATGP3933-10 µg, ATGP3933-20 µg, ATGP3933-50 µg, ATGP3933-100 µg, ATGP3933-250 µg, ATGP3933-500 µg, ATGP3933-1 mg, ATGP3933-10, ATGP3933-20, ATGP3933-50, ATGP3933-100, ATGP3933-250, ATGP3933-500, ATGP3933-1

References & Citations

Kolter, T. et al. (2002) J. Biol. Chem. 277:25859-25862.; ; Okajima, T. et al. (1999) J. Biol. Chem.274:30557-30562.; ; Harduin-Lepers, A. et al. (2005) Glycobiology 15:805-817.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Tags & Cell Markers

NCBI Accession Number

NP_112227

Uniprot Accession Number

Q9BVH7

Species

Human

N Terminal Sequence

Ala39

AA Sequence

GGQKERP PQQQQQQQQQ QQQASATGSS QPAAESSTQQ RPGVPAGPRP LDGYLGVADH KPLKMHCRDC ALVTSSGHLL HSRQGSQIDQ TECVIRMNDA PTRGYGRDVG NRTSLRVIAH SSIQRILRNR HDLLNVSQGT VFIFWGPSSY MRRDGKGQVY NNLHLLSQVL PRLKAFMITR HKMLQFDELF KQETGKDRKI SNTWLSTGWF TMTIALELCD RINVYGMVPP DFCRDPNHPS VPYHYYEPFG PDECTMYLSH ERGRKGSHHR FITEKRVFKN WARTFNIHFF QPDWKPESLA INHPENKPVF