Recombinant mouse IL-1 alpha/IL1A protein
IL-1 alpha /IL-1F1, also known as interleukin-1 alpha, is a member of the interleukin-1 cytokine family. Cytokines are protein signaling compounds that are the main mediators of the immune response. This protein is produced mainly by activated macrophages, as well as neutrophils, epithelial cells, and endothelial cells. It possesses metabolic, physiological, haematopoietic activities, and plays one of the central roles in the regulation of the immune responses. It binds to the interleukin-1 receptor. It is on the pathway that activates tumor necrosis factor-alpha. Also, it stimulates thymocyte proliferation by inducing IL-2 release, B cell maturation and proliferation. Recombinant mouse IL-1 alpha, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Mouse IL-1 alpha /IL-1F1 stimulates cell proliferation of the D10.G4.1 mouse helper T cells. The ED50 range ≤ 0.15 ng/mL.
Product Name Alternative
Interleukin-1 alpha precursor, Interleukin 1 alpha, IL-1F1, IL-1A, IL-1 alpha
Expression System
Baculovirus
Antigen Species
Mouse
Tag
His-Tag
Applications
SDS-PAGE, Bioactivity
Concentration
0.5 mg/mL (determined by Absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 90% by SDS-PAGE
Bioactivity
Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED50 range ≤ 0.15 ng/mL.
Molecular Weight
19.0kDa (165aa)
Additionnal Information
References & Citations
Dinarello CA., et al. (1997) Semin Oncol. 24:S9-81-S9-93.; ; Bankers-Fulbright JL., et al. (1996) Life Sci. 59:61-83.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Scientific Category
Immunology
NCBI Accession Number
NP_034684
Uniprot Accession Number
P01582
Species
Mouse
N Terminal Sequence
Ala
AA Sequence
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SKAP2, ID (SKAP2, PRAP, RA70, SAPS, SCAP2, SKAP55R, Src kinase-associated phosphoprotein 2, Pyk2/RAFTK-associated protein, Retinoic acid-induced protein 70, SKAP55 homolog, Src family-associated phosphoprotein 2, Src kinase-associated phosphoprotein 55-re
