Recombinant mouse IL-1 alpha/IL1A protein

IL-1 alpha /IL-1F1, also known as interleukin-1 alpha, is a member of the interleukin-1 cytokine family. Cytokines are protein signaling compounds that are the main mediators of the immune response. This protein is produced mainly by activated macrophages, as well as neutrophils, epithelial cells, and endothelial cells. It possesses metabolic, physiological, haematopoietic activities, and plays one of the central roles in the regulation of the immune responses. It binds to the interleukin-1 receptor. It is on the pathway that activates tumor necrosis factor-alpha. Also, it stimulates thymocyte proliferation by inducing IL-2 release, B cell maturation and proliferation. Recombinant mouse IL-1 alpha, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Mouse IL-1 alpha /IL-1F1 stimulates cell proliferation of the D10.G4.1 mouse helper T cells. The ED50 range ≤ 0.15 ng/mL.

Product Name Alternative

Interleukin-1 alpha precursor, Interleukin 1 alpha, IL-1F1, IL-1A, IL-1 alpha

Expression System

Baculovirus

Antigen Species

Mouse

Tag

His-Tag

Applications

SDS-PAGE, Bioactivity

Concentration

0.5 mg/mL (determined by Absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Bioactivity

Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED50 range ≤ 0.15 ng/mL.

Molecular Weight

19.0kDa (165aa)

Additionnal Information

ATGP3778, Pro-interleukin-1-alpha, Pro interleukin 1 alpha, Preinterleukin 1 alpha, Interleukin-1 alpha precursor, Interleukin 1 alpha, IL1F1, IL-1a, IL1a, IL-1 alpha, IL1 alpha, IL-1 a, IL1 a, IL 1 alpha, IL 1 a, Hematopoietin-1, Hematopoietin1, Hematopoietin 1, ATGP3932-10 µg, ATGP3932-20 µg, ATGP3932-50 µg, ATGP3932-100 µg, ATGP3932-250 µg, ATGP3932-500 µg, ATGP3932-1 mg, ATGP3932-010, ATGP3932-020, ATGP3932-050, ATGP3932-100, ATGP3932-250, ATGP3932-500, ATGP3932-01M, ATGP3778

References & Citations

Dinarello CA., et al. (1997) Semin Oncol. 24:S9-81-S9-93.; ; Bankers-Fulbright JL., et al. (1996) Life Sci. 59:61-83.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Immunology

NCBI Accession Number

NP_034684

Uniprot Accession Number

P01582

Species

Mouse

N Terminal Sequence

Ala

AA Sequence

SAPYTYQ SDLRYKLMKL VRQKFVMNDS LNQTIYQDVD KHYLSTTWLN DLQQEVKFDM YAYSSGGDDS KYPVTLKISD SQLFVSAQGE DQPVLLKELP ETPKLITGSE TDLIFFWKSI NSKNYFTSAA YPELFIATKE QSRVHLARGL PSMTDFQIS