What is the catalog number for Recombinant human ICAM-3/CD50 protein?

The catalog number for Recombinant human ICAM-3/CD50 protein is ATGP3931-100.

What sizes are available for Recombinant human ICAM-3/CD50 protein?

Recombinant human ICAM-3/CD50 protein is available in the following size(s): 100 µg.

Is Recombinant human ICAM-3/CD50 protein in stock?

Recombinant human ICAM-3/CD50 protein is currently made to order / not held in immediate stock. Contact us for lead time.

Recombinant human ICAM-3/CD50 protein

ICAM-3/CD50, also known as CD50 (Cluster of Differentiation 50), is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. This protein is constitutively and abundantly expressed by all leukocytes and may be the most important ligand for LFA-1 in the initiation of the immune response. It functions not only as an adhesion molecule, but also as a potent signaling molecule. Recombinant human ICAM-3/CD50, fused hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Human ICAM-3/CD50 stimulates cell adhesion of the HL-60 human promyelocytic cells. The ED50 range ≤4 µg/mL.

Product Name Alternative

ICAM3, Intercellular adhesion molecule 3, ICAM-3, CD50, CDW50, ICAM-R, intercellular adhesion molecule 3 isoform 1

Expression System

Baculovirus

Antigen Species

Human

Tag

HIgG-His-Tag

Applications

SDS-PAGE, Bioactivity

Concentration

0.25 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Bioactivity

Measured by the ability of the immobilized protein to support the adhesion of HL-60 human promyelocytic cells. When cells are added to Human ICAM-3/CD50 coated plates, the ED50 range ≤4 µg/mL.

Molecular Weight

76.7 kDa (698aa)

Additionnal Information

ICAM3, CD50, ATGP3931-10 µg, ATGP3931-20 µg, ATGP3931-50 µg, ATGP3931-100 µg, ATGP3931-250 µg, ATGP3931-500 µg, ATGP3931-1 mg, ATGP3931-10, ATGP3931-20, ATGP3931-50, ATGP3931-100, ATGP3931-250, ATGP3931-500, ATGP3931-1

References & Citations

De Fougerolles AR., et al, (1992) J Exp Med. 175:185-190.; ; Serrador JM., et al, (1997) J Cell Biol. 138:1409-1423.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Signal Transduction

NCBI Accession Number

NP_002153.2

Uniprot Accession Number

P32942

Species

Human

N Terminal Sequence

Ala

AA Sequence

QEFLLRV EPQNPVLSAG GSLFVNCSTD CPSSEKIALE TSLSKELVAS GMGWAAFNLS NVTGNSRILC SVYCNGSQIT GSSNITVYRL PERVELAPLP PWQPVGQNFT LRCQVEDGSP RTSLTVVLLR WEEELSRQPA VEEPAEVTAT VLASRDDHGA PFSCRTELDM QPQGLGLFVN TSAPRQLRTF VLPVTPPRLV APRFLEVETS WPVDCTLDGL FPASEAQVYL ALGDQMLNAT VMNHGDTLTA TATATARADQ EGAREIVCNV TLGGERREAR ENLTVFSFLG PIVNLSEPTA HEGSTVTVSC MAGARVQVTL DGVPAAAPGQ PAQLQLNATE SDDGRSFFCS ATLEVDGEFL HRNSSVQLRV LYGPKIDRAT CPQHLKWKDK TRHVLQCQAR GNPYPELRCL KEGSSREVPV GIPFFVNVTH NGTYQCQASS SRGKYTLVVV MDIEAGSSH