Recombinant mouse Thrombopoietin protein
Thrombopoietin (THPO), also known as megakaryocyte growth and development factor (MGDF), is a glycoprotein hormone produced by the liver and kidney which regulates the production of platelets. It stimulates the production and differentiation of megakaryocytes, the bone marrow cells that bud off large numbers of platelets. Megakaryocytopoiesis is the cellular development process that leads to platelet production. It is a humoral growth factor necessary for megakaryocyte proliferation and maturation, as well as for thrombopoiesis. This protein is the ligand for MLP/C_MPL, the product of myeloproliferative leukemia virus oncogene. Recombinant mouse Thrombopoietin, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Mouse Thrombopoietin stimulates cell proliferation of the MO7e human megakaryocytic leukemic cells. The ED50 range ≤4 ng/mL.
Product Name Alternative
Expression System
HEK293
Antigen Species
Mouse
Tag
His-Tag
Applications
SDS-PAGE, Bioactivity
Concentration
0.5 mg/mL (determined by absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 90% by SDS-PAGE
Bioactivity
Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED50 range ≤4 ng/mL.
Molecular Weight
36.4 kDa (341aa)
Additionnal Information
References & Citations
Jenkins JM., et al. (2007) Blood. 109:4739-4741.; ; Hitchcock IS., et al. (2014) Br J Haemato. 165:259-268.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Scientific Category
Cardiovascular
NCBI Accession Number
NP_033405
Uniprot Accession Number
P40226
Species
Mouse
N Terminal Sequence
Ser22
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