Recombinant firefly Luciferase protein
Luciferase is a generic term for the class of oxidative enzymes used in bioluminescence and is distinct from a photoprotein. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP, produces green light with a wavelength of 562 nm. Luciferase from firefly is widely used as a reporter for studying gene regulation and function, and for pharmaceutical screening. Recombinant firefly Luciferase, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Specific activity is 1X10^10 light units/mg. One luciferase enzyme units will produce one Relative Light Unit (RLU) at 25C
Product Name Alternative
Luciferin 4-monooxygenase, LuC
Expression System
E.coli
Antigen Species
Firefly
Tag
His-Tag
Applications
SDS-PAGE, Enzyme Activity
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Bioactivity
Specific activity is 1X10^10 lightunit/mg. One luciferase enzymeunit will produce one Relative Light Unit (RLU) at 25C
Molecular Weight
63 kDa (571aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Moore S A., et al. (1995) J Mol Biol. 249 (1) :195-214; ; Gould S J., et al. (1988) Anal Biochem. 175 (1) :5-13.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol
Scientific Category
Enzymes & Proteases
NCBI Accession Number
AB261982
Uniprot Accession Number
A3KBZ5
Species
Firefly
AA Sequence
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