What is the catalog number for Recombinant firefly Luciferase protein?

The catalog number for Recombinant firefly Luciferase protein is ATGP3928-010.

What sizes are available for Recombinant firefly Luciferase protein?

Recombinant firefly Luciferase protein is available in the following size(s): 10 µg.

Is Recombinant firefly Luciferase protein in stock?

Recombinant firefly Luciferase protein is currently made to order / not held in immediate stock. Contact us for lead time.

Recombinant firefly Luciferase protein

Luciferase is a generic term for the class of oxidative enzymes used in bioluminescence and is distinct from a photoprotein. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP, produces green light with a wavelength of 562 nm. Luciferase from firefly is widely used as a reporter for studying gene regulation and function, and for pharmaceutical screening. Recombinant firefly Luciferase, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is 1X10^10 light units/mg. One luciferase enzyme units will produce one Relative Light Unit (RLU) at 25C

Product Name Alternative

Luciferin 4-monooxygenase, LuC

Expression System

E.coli

Antigen Species

Firefly

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Bioactivity

Specific activity is 1X10^10 lightunit/mg. One luciferase enzymeunit will produce one Relative Light Unit (RLU) at 25C

Molecular Weight

63 kDa (571aa) confirmed by MALDI-TOF

Additionnal Information

Luciferase, ATGP3928-10 µg, ATGP3928-20 µg, ATGP3928-50 µg, ATGP3928-100 µg, ATGP3928-250 µg, ATGP3928-500 µg, ATGP3928-1 mg, ATGP3928-10, ATGP3928-20, ATGP3928-50, ATGP3928-100, ATGP3928-250, ATGP3928-500, ATGP3928-1

References & Citations

Moore S A., et al. (1995) J Mol Biol. 249 (1) :195-214; ; Gould S J., et al. (1988) Anal Biochem. 175 (1) :5-13.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol

Scientific Category

Enzymes & Proteases

NCBI Accession Number

AB261982

Uniprot Accession Number

A3KBZ5

Species

Firefly

AA Sequence

MEDAKNIKK GPAPFYPLED GTAGEQLHKA MKRYALVPGT IAFTDAHIEV NITYAEYFEM SVRLAEAMKR YGLNTNHRIV VCSENSLQFF MPVLGALFIG VAVAPANDIY NERELLNSMN ISQPTVVFVS KKGLQKILNV QKKLPIIQKI IIMDSKTDYQ GFQSMYTFVT SHLPPGFNEY DFVPESFDRD KTIALIMNSS GSTGLPKGVA LPHRTACVRF SHARDPIFGN QIIPDTAILS VVPFHHGFGM FTTLGYLICG FRVVLMYRFE EELFLRSLQD YKIQSALLVP TLFSFFAKST LIDKYDLSNL HEIASGGAPL SKEVGEAVAK RFHLPGIRQG YGLTETTSAI LITPEGDDKP GAVGKVVPFF EAKVVDLDTG KTLGVNQRGE LCVRGPMIMS GYVNNPEATN ALIDKDGWLH SGDLAYWDED EHFFIVDRLK SLIKYKGYQV APAELESILL QHPNIFDAGV AGLPDDDAGE LPAAVVVLEH GKTMTEKEIV DYVASQVTTA KKLRGGVVFV DEVPKGLTGK LDARKIREIL IKAKKGGKSK L