Recombinant human Kallikrein 3/PSA protein

Kallikrein 3, as known as prostate specific antigen (PSA), is a subgroup of serine proteases, enzymes capable of cleaving peptide bonds in proteins. This protein is expressed in the prostate and is thought to be responsible for regulating semen liquefaction through hydrolysis of seminogelin. It is present in small quantities in the serum of men with healthy prostates, but is often elevated in the presence of prostate cancer or other prostate disorders. It is not a unique indicator of prostate cancer, but may also detect prostatitis or benign prostatic hyperplasia. Recombinant human Kallikrein 3 protein, fused to His-tag at C-terminus, was expressed in HEK293 and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is > 250 pmol/min/ug, and is defined as the amount of enzyme cleaves 1pmole of Succinyl-Arg-Pro-Tyr-p-Nitroanilide to Succinyl-Arg-Pro-Tyr and p-Nitroanilide per minute at pH7.5 at 37C.

Product Name Alternative

KLK3, Prostate-specific antigen, PSA, Gamma-seminoprotein, Seminin, Kallikrein-3, P-30 antigen, Semenogelase, APS, hK3, KLK2A1, kallikrein related peptidase 3, prostate-specific antigen isoform 1

Expression System

HEK293

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

0.25 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Bioactivity

Specific activity is > 250pmol/min/ug, and is defined as the amount of enzyme cleaves 1pmole of Succinyl-Arg-Pro-Tyr-p-Nitroanilide to Succinyl-Arg-Pro-Tyr and p-Nitroanilide per minute at pH7.5 at 37C.

Molecular Weight

27.6 kDa (250aa)

Additionnal Information

Seminin, Semenogelase, PSA, Prostate-specific antigen isoform 1, Prostate-specific antigen, Prostate specific antigen isoform 1, Prostate specific antigen, P-30 antigen, P30 antigen, KLK3, KLK2A1, Kallikrein-3, Kallikrein3, Kallikrein related peptidase 3, Kallikrein 3, hK3, Gamma-seminoprotein, APS, Gamma seminoprotein, ATGP3926-10 µg, ATGP3926-20 µg, ATGP3926-50 µg, ATGP3926-100 µg, ATGP3926-250 µg, ATGP3926-500 µg, ATGP3926-1 mg, ATGP3926-010, ATGP3926-020, ATGP3926-050, ATGP3926-100, ATGP3926-250, ATGP3926-500, ATGP3926-01M