Recombinant human VAP-1/AOC3 protein
VAP-1, also known as membrane primary amine oxidase isoform 1, a copper amine oxidase with a topaquinone cofactor. It is a cell adhesion protein that participates in lymphocyte extravasation and recirculation by mediating the binding of lymphocytes to peripheral lymph node vascular endothelial cells in an L-selectin-independent fashion. This protein plays a role in adipogenesis. It catalyzes the oxidative deamination of small primary amines such as methylamine, benzylamine, and aminoacetone in a reaction that produces an aldehyde, ammonia, and H2O2. Recombinant human VAP-1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Expression System
Baculovirus
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 90% by SDS-PAGE
Molecular Weight
82.8 kDa (746aa)
Additionnal Information
References & Citations
Hsia LT., et al. (2016) Proc Natl Acad Sci U S A. 113:E2162-71; ; Trivedi PJ., et al. (2018) Gut. 67:1135-1145
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Scientific Category
Neuroscience
NCBI Accession Number
NP_003725
Uniprot Accession Number
Q16853
Species
Human
N Terminal Sequence
Ala
AA Sequence
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