Recombinant human RAGE/AGER protein
RAGE, also known as advanced glycosylation end product-specific receptor isoform 1, is a member of the immunoglobulin super-family transmembrane proteins. It mediates interactions of advanced glycosylation end products (AGE) . AGE/RAGE signaling plays an important role in regulating the production/expression of TNF-alpha, oxidative stress, and endothelial dysfunction in type 2 diabetes. This protein contributes to the translocation of amyloid-beta peptide (ABPP) across the cell membrane from the extracellular to the intracellular space in cortical neurons. Recombinant human RAGE, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Human HMGB1 is coated at 20 µg/mL (100 ul/well) can bind Human RAGE. The ED50 range ≤15 µg/mL.
Product Name Alternative
RAGE, AGER, advanced glycosylation end product-specific receptor isoform 1, SCARJ1, Receptor for advanced glycosylation end products
Expression System
Baculovirus
Antigen Species
Human
Tag
HIgG-His-Tag
Applications
SDS-PAGE, Bioactivity
Concentration
0.25 mg/mL (determined by BCA assay)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 90% by SDS-PAGE
Bioactivity
Measured by the ability of the binding activity in a functional ELISA. The ED50 range ≤15 µg/mL.
Molecular Weight
61.2 kDa (561aa)
Additionnal Information
References & Citations
Giurdanella G., et al. (2017) Biochem Pharmacol. 142:145-154; ; Zhang L., et al. (2019) Int J Mol Med. 44:885-892
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Scientific Category
Neuroscience
NCBI Accession Number
NP_001127
Uniprot Accession Number
Q15109
Species
Human
N Terminal Sequence
Ala
AA Sequence
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