Recombinant human RAGE/AGER protein

RAGE, also known as advanced glycosylation end product-specific receptor isoform 1, is a member of the immunoglobulin super-family transmembrane proteins. It mediates interactions of advanced glycosylation end products (AGE) . AGE/RAGE signaling plays an important role in regulating the production/expression of TNF-alpha, oxidative stress, and endothelial dysfunction in type 2 diabetes. This protein contributes to the translocation of amyloid-beta peptide (ABPP) across the cell membrane from the extracellular to the intracellular space in cortical neurons. Recombinant human RAGE, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Human HMGB1 is coated at 20 µg/mL (100 ul/well) can bind Human RAGE. The ED50 range ≤15 µg/mL.

Product Name Alternative

RAGE, AGER, advanced glycosylation end product-specific receptor isoform 1, SCARJ1, Receptor for advanced glycosylation end products

Expression System

Baculovirus

Antigen Species

Human

Tag

HIgG-His-Tag

Applications

SDS-PAGE, Bioactivity

Concentration

0.25 mg/mL (determined by BCA assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Bioactivity

Measured by the ability of the binding activity in a functional ELISA. The ED50 range ≤15 µg/mL.

Molecular Weight

61.2 kDa (561aa)

Additionnal Information

RAGE, ATGP3923-10 µg, ATGP3923-20 µg, ATGP3923-50 µg, ATGP3923-100 µg, ATGP3923-250 µg, ATGP3923-500 µg, ATGP3923-1 mg, ATGP3923-10, ATGP3923-20, ATGP3923-50, ATGP3923-100, ATGP3923-250, ATGP3923-500, ATGP3923-1

References & Citations

Giurdanella G., et al. (2017) Biochem Pharmacol. 142:145-154; ; Zhang L., et al. (2019) Int J Mol Med. 44:885-892

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Neuroscience

NCBI Accession Number

NP_001127

Uniprot Accession Number

Q15109

Species

Human

N Terminal Sequence

Ala

AA Sequence

QNITARI GEPLVLKCKG APKKPPQRLE WKLNTGRTEA WKVLSPQGGG PWDSVARVLP NGSLFLPAVG IQDEGIFRCQ AMNRNGKETK SNYRVRVYQI PGKPEIVDSA SELTAGVPNK VGTCVSEGSY PAGTLSWHLD GKPLVPNEKG VSVKEQTRRH PETGLFTLQS ELMVTPARGG DPRPTFSCSF SPGLPRHRAL RTAPIQPRVW EPVPLEEVQL VVEPEGGAVA PGGTVTLTCE VPAQPSPQIH WMKDGVPLPL PPSPVLILPE IGPQDQGTYS CVATHSSHGP QESRAVSISI IEPGEEGPTA GSVGGSGLGT LA