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Recombinant human Cytochrome p450 reductase/POR protein

POR, also known as NADPH--cytochrome P450 reductase, is a flavoprotein that donates electrons to all microsomal P450 enzymes. POR is localized to the endoplasmic reticulum, where it is also able to transfer electrons to heme oxygenase and cytochrome b5. It is structurally related to two separate flavoprotein families, ferredoxin nucleotide reductase and flavodoxin. Recombinant human POR, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Cytochrome p450 oxidoreductase, NADPH--cytochrome P450 reductase, NADPH--hemoprotein reductase, CYPOR, CPR, P450R

Expression System

Baculovirus

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Molecular Weight

77.9 kDa (686aa)

Additionnal Information

POR, Cytochrome p450 oxidoreductase, NADPH--cytochrome P450 reductase, NADPH--hemoprotein reductase, CYPOR, CPR, P450R, NADPH-cytochrome P450 reductase, NADPH-hemoprotein reductase, NADPH cytochrome P450 reductase, NADPH hemoprotein reductase, ATGP3921-10 µg, ATGP3921-20 µg, ATGP3921-50 µg, ATGP3921-100 µg, ATGP3921-250 µg, ATGP3921-500 µg, ATGP3921-1 mg, ATGP3921-010, ATGP3921-020, ATGP3921-050, ATGP3921-100, ATGP3921-250, ATGP3921-500, ATGP3921-01M

References & Citations

Shen A L, et al. (1989) . J Biol Chem. 264:7584-7589.; ; Haniju M, et al. (1989) . Biochemistry. 28:8639-8645.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Cardiovascular

NCBI Accession Number

NP_000932

Uniprot Accession Number

P16435

Species

Human

N Terminal Sequence

Met1

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