Recombinant human IL-10 protein

IL-10, also known as Interleukin-10, is an anti-inflammatory cytokine. It inhibits the synthesis of pro-inflammatory cytokines like IFN-gamma, IL2, IL3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. This protein has effects in immunoregulation and inflammation and down-regulates the expression of Th1 cytokines, MHC class II antigens, and costimulatory molecules on macrophages. It acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. This protein controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling. Recombinant human IL-10, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Human IL-10 stimulates cell proliferation of the MC/9 mouse mast cells. The ED50 range ≤5 ng/mL.

Product Name Alternative

Interleukin-10, IL-10, Cytokine synthesis inhibitory factor, CSIF, GVHDS, IL10A, TGIF, T-cell growth inhibitory factor

Expression System

Baculovirus

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Bioactivity

Concentration

0.5 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Bioactivity

Measured in a cell proliferation assay using MC/9 mouse mast cells. The ED50 range ≤5 ng/mL.

Molecular Weight

19.4 kDa (166aa)

Additionnal Information

Interleukin10, Interleukin 10, Interleukin-10, IL-10, IL10, IL 10, Cytokine synthesis inhibitory factor, CSIF, GVHDS, IL10A, TGIF, T-cell growth inhibitory factor, ATGP3920-10 µg, ATGP3920-20 µg, ATGP3920-50 µg, ATGP3920-100 µg, ATGP3920-250 µg, ATGP3920-500 µg, ATGP3920-1 mg, ATGP3920-010, ATGP3920-020, ATGP3920-050, ATGP3920-100, ATGP3920-250, ATGP3920-500, ATGP3920-01M

References & Citations

Jog NR., et al. (2018) Front Immunol. 9:2198.; ; Harling K., et al. (2019) Cell Mol Immunol. 16:275-287.

Other References

Lee HM, et al. A comparative study of the effects of inhibitory cytokines on human natural killer cells and the mechanistic features of transforming growth factor-beta. (Cell Immunol. 2014) {https://pubmed.ncbi.nlm.nih.gov/24879062/}