Recombinant human IL-6R alpha/IL6R protein

IL-6R alpha, also known as interleukin-6 receptor subunit alpha isoform 1, is a type I cytokine receptor. It is a potent pleiotropic cytokine that regulates cell growth and differentiation and plays an important role in immune response. It has a modular build of several immunoglobulin-like and fibronectin type III-like domains. The low concentration of a soluble form of IL-6 receptor (sIL-6R) acts as an agonist of IL-6 activity. In the IL-6R/CD126/IL6R system, both a membrane-bound IL-6R and a sIL-6R protein are able to mediate IL-6 signals into the cells through the interaction of gp130. It is implicated in the pathogenesis of many diseases, such as multiple myeloma, autoimmune diseases and prostate cancer. Recombinant human IL-6R alpha, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Human IL-6 R alpha inhibits Human IL-6 induced cell proliferation in the M1 mouse myeloid leukemia cells. The ED50 range ≤20 ng/mL.

Product Name Alternative

Interleukin-6 receptor subunit alpha, IL-6 receptor subunit alpha, IL-6R subunit alpha, IL-6R 1, Membrane glycoprotein 80, gp80, CD126

Expression System

Baculovirus

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Bioactivity

Concentration

0.25 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Bioactivity

Measured by its ability to inhibit proliferation using M1 mouse myeloid leukemia cells. The ED50 range ≤20 ng/mL with Human IL-6.

Molecular Weight

39.6 kDa (355aa)

Additionnal Information

IL6R a, ATGP3919-10 µg, ATGP3919-20 µg, ATGP3919-50 µg, ATGP3919-100 µg, ATGP3919-250 µg, ATGP3919-500 µg, ATGP3919-1 mg, ATGP3919-10, ATGP3919-20, ATGP3919-50, ATGP3919-100, ATGP3919-250, ATGP3919-500, ATGP3919-1

References & Citations

Sugita T., et al. (1990) J Exp Med. 171:2001-2009.; ; Yamasaki K., et al. (1988) Science. 241:825-828.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Hormones & Cytokines

NCBI Accession Number

NP_000556

Uniprot Accession Number

P08887

Species

Human

N Terminal Sequence

Ala

AA Sequence

LAPRRCP AQEVARGVLT SLPGDSVTLT CPGVEPEDNA TVHWVLRKPA AGSHPSRWAGMGRRLLLRSV QLHDSGNYSC YRAGRPAGTV HLLVDVPPEE PQLSCFRKSP LSNVVCEWGPRSTPSLTTKA VLLVRKFQNS PAEDFQEPCQ YSQESQKFSC QLAVPEGDSS FYIVSMCVASSVGSKFSKTQ TFQGCGILQP DPPANITVTA VARNPRWLSV TWQDPHSWNS SFYRLRFELRYRAERSKTFT TWMVKDLQHH CVIHDAWSGL RHVVQLRAQE EFGQGEWSEW SPEAMGTPWTESRSPPAENE VSTPMQALTT NKDDDNILFR DSANATSLPV QDSSSVPLP