Recombinant human BACE-1 protein

BACE-1, also known as beta-secretase 1 isoform A, is a member of aspartic protease and an integral membrane protein. It is involved in the proteolytic processing of the amyloid precursor protein (APP) . This protein cleaves at the N-terminus of the A-beta peptide sequence of APP, and then leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase. It has been implicated in the onset and/or progression of Alzheimer's disease. It is also distantly related to the pathogenic aspartic-acid protease plasmepsin, which is a potential target for future anti-malarial drugs. Recombinant human BACE-1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is > 5 pmol/min/ug in which one unit will convert 1.0 pmole of Mca-SEVNLDAEFRK (Dnp) RR-NH2 to MCA- Pro-Leu-OH per minute at pH 3.5 at 25C.

Product Name Alternative

BACE1, Beta-secretase 1, Aspartyl protease 2, ASP2, Asp 2, Beta-site amyloid precursor protein cleaving enzyme 1, Beta-site APP cleaving enzyme 1, Memapsin-2, Membrane-associated aspartic protease 2, BACE, KIAA1149, HSPC104

Expression System

Baculovirus

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

0.5 mg/mL (determined by BCA assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Bioactivity

Specific activity is > 5pmol/min/ug in which one unit will convert 1.0pmole of Mca-SEVNLDAEFRK (Dnp) RR-NH2 to MCA- Pro-Leu-OH per minute at pH 3.5 at 25C.

Molecular Weight

49.2 kDa (442aa)

Additionnal Information

BACE1, ATGP3917-10 µg, ATGP3917-20 µg, ATGP3917-50 µg, ATGP3917-100 µg, ATGP3917-250 µg, ATGP3917-500 µg, ATGP3917-1 mg, ATGP3917-10, ATGP3917-20, ATGP3917-50, ATGP3917-100, ATGP3917-250, ATGP3917-500, ATGP3917-1