What is the catalog number for Recombinant mouse TRANCE/RANKL/TNFSF11 protein?

The catalog number for Recombinant mouse TRANCE/RANKL/TNFSF11 protein is ATGP3915-100.

What sizes are available for Recombinant mouse TRANCE/RANKL/TNFSF11 protein?

Recombinant mouse TRANCE/RANKL/TNFSF11 protein is available in the following size(s): 100 µg.

Is Recombinant mouse TRANCE/RANKL/TNFSF11 protein in stock?

Recombinant mouse TRANCE/RANKL/TNFSF11 protein is currently made to order / not held in immediate stock. Contact us for lead time.

Recombinant mouse TRANCE/RANKL/TNFSF11 protein

Tnfsf11, also known as tumor necrosis factor ligand superfamily member 11, is a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. Tnfsf11 also has a function in the immune system, where it is expressed by T helper cells and is thought to be involved in dendritic cell maturation. Tnfsf11 is important in bone metabolism. This natural and necessary surface-bound molecule (also known as CD254) found on osteoblasts serves to activate osteoclasts, which are the cells involved in bone resorption. Recombinant Mouse Tnfsf11 protein was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Specifications

Mouse TRANCE/RANK L/TNFSF11 stimulates osteoclast differentiation of the RAW 264.7 mouse monocyte/macrophage cells. The ED50 range ≤2 ng/mL.

Product Name Alternative

Tumor necrosis factor ligand superfamily member 11, Osteoclast differentiation factor, ODF, Osteoprotegerin ligand, OPGL, Receptor activator of nuclear factor kappa-B ligand, RANKL, TNF-related activation-induced cytokine, TRANCE, CD254

Expression System

E.coli

Antigen Species

Mouse

Tag

Non-Tagged

Applications

SDS-PAGE, Bioactivity

Concentration

1 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Bioactivity

Measured in a osteoclast differentiation assay using RAW 264.7 mouse monocyte/macrophage cells. The ED50 range ≤2 ng/mL.

Molecular Weight

17.9 kDa (160aa) confirmed by MALDI-TOF

Additionnal Information

TRANCE, RANK L, TNFSF11, ATGP3915-10 µg, ATGP3915-20 µg, ATGP3915-50 µg, ATGP3915-100 µg, ATGP3915-250 µg, ATGP3915-500 µg, ATGP3915-1 mg, ATGP3915-10, ATGP3915-20, ATGP3915-50, ATGP3915-100, ATGP3915-250, ATGP3915-500, ATGP3915-1

References & Citations

Lam J., et al. (2001) . J. Clin. Invest. 108 (7) :971-9.; ; Ito S., et al. (2002) . J. Biol. Chem. 277 (8) :6631-6.

Other References

Park KH, et al. Zinc inhibits osteoclast differentiation by suppression of Ca2+-Calcineurin-NFATc1 signaling pathway. (Cell Commun Signal. 2013) {https://pubmed.ncbi.nlm.nih.gov/24088289/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid In. 20 mM Tris-HCl buffer (pH8.5) containing 0.1M NaCl

Scientific Category

Hormones & Cytokines

NCBI Accession Number

NP_035743

Uniprot Accession Number

O35235

Species

Mouse

AA Sequence

MKPEAQPFAH LTINAASIPS GSHKVTLSSW YHDRGWAKIS NMTLSNGKLR VNQDGFYYLY ANICFRHHET SGSVPTDYLQ LMVYVVKTSI KIPSSHNLMK GGSTKNWSGN SEFHFYSINV GGFFKLRAGE EISIQVSNPS LLDPDQDATY FGAFKVQDID