Recombinant human CTLA4 protein

CTLA-4, also known as cytotoxic T-lymphocyte protein 4, is a type I transmembrane T cell inhibitory molecule that is a member of the Ig superfamily. The affinity of CTLA-4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28. Recombinant human CTLA-4, fused to hIgG His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Human CTLA-4 inhibits Human B7 1/CD80 induced IL-2 secretion of the Jurkat human acute T cell leukemia cells. The ED50 range ≤150 ng/mL.

Product Name Alternative

Cytotoxic T-lymphocyte protein 4 isoform CTLA4-TM precursor, Cytotoxic T-lymphocyte-associated antigen 4, CTLA-4, CD152, CELIAC3, Celiac disease 3, Insulin-dependent diabetes mellitus 12, IDDM12, CD, GSE

Expression System

Baculovirus

Antigen Species

Human

Tag

HIgG-His-Tag

Applications

SDS-PAGE, Bioactivity

Concentration

0.5 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Bioactivity

The activity is determined by the IL-2 ELISA in a using Jurkat human acute T cell leukemia cells. The ED50 range ≤150 ng/mL with Human B7 1/CD80.

Molecular Weight

40.8 kDa (368aa)

Additionnal Information

Cytotoxic T lymphocyte protein 4 isoform CTLA4-TM precursor, Cytotoxic T-lymphocyte-associated antigen 4, CTLA 4, CTLA4, CTLA-4, CD 152, CD152, CELIAC3, CELIAC 3, Celiac disease 3, Insulin-dependent diabetes mellitus 12, Insulin dependent diabetes mellitus 12, IDDM 12, IDDM12, CD, GSE, ATGP3914-10 µg, ATGP3914-20 µg, ATGP3914-50 µg, ATGP3914-100 µg, ATGP3914-250 µg, ATGP3914-500 µg, ATGP3914-1 mg, ATGP3914-010, ATGP3914-020, ATGP3914-050, ATGP3914-100, ATGP3914-250, ATGP3914-500, ATGP3914-01M, ATGP3364