Recombinant mouse Granzyme B protein

Granzyme B, also known as Gzmb, is a member of the granzyme family of cell death-inducing serine proteases specially expressed in the granules of cytotoxic T lymphocytes (CTLs) and NK cells. It plays crucial role for target cell lysis in cell-mediated immune responses. This protein is linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. It cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis. Recombinant mouse Granzyme B, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is > 9,000 pmol/min/ug, and is defined as the amount of enzyme that cleave 1pmole of Boc-Ala-Ala-Asp-SBzl at 37C.

Product Name Alternative

Gzmb, CTLA-1, Cytotoxic cell protease 1, CCP1, Fragmentin-2, Ctla-1, Ctla1, AI553453, CCP-1/C11, CCP1, GZB

Expression System

Baculovirus

Antigen Species

Mouse

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

0.25 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Bioactivity

Specific activity is > 9,000pmol/min/ug, and is defined as the amount of enzyme that cleave 1pmole of Boc-Ala-Ala-Asp-SBzl at 37C.

Molecular Weight

26.3 kDa (235aa)

Additionnal Information

Granzyme B, ATGP3913-10 µg, ATGP3913-20 µg, ATGP3913-50 µg, ATGP3913-100 µg, ATGP3913-250 µg, ATGP3913-500 µg, ATGP3913-1 mg, ATGP3913-10, ATGP3913-20, ATGP3913-50, ATGP3913-100, ATGP3913-250, ATGP3913-500, ATGP3913-1