Recombinant human IL-9 protein
IL-9, also known as interleukin 9, is a cytokine (cell signalling molecule) belonging to the group ofinterleukins. This protein produced by T-cells and specifically by CD4+ helper cells that acts as a regulatorof a variety of hematopoietic cells and stimulates cell proliferation and prevents apoptosis. It functions throughthe interleukin-9 receptor (IL9R), which activates different signal transducer and activator (STAT) proteins and thus connects this cytokine to various biological processes. Recombinant human IL-9, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Human IL-9 stimulates cell proliferation of the MO7e human megakaryocytic leukemic cells. The ED50 range ≤0.3 ng/mL.
Product Name Alternative
Expression System
Baculovirus
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE, Bioactivity
Concentration
0.5 mg/mL (determined by BCA assay)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 85% by SDS-PAGE
Bioactivity
Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED50 range ≤0.3 ng/mL.
Molecular Weight
14.9 kDa (132aa)
Additionnal Information
References & Citations
Purwar R, Schlapbach C, et al. (2012) . Nat Med. 18 (8) :1248-53. ; ; Pilette C, Ouadrhiri Y, et al. (2002) . J Immunol. 168 (8) :4103-11.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Scientific Category
Hormones & Cytokines
NCBI Accession Number
NP_000581
Uniprot Accession Number
P15248
Species
Human
N Terminal Sequence
Gln19
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