Recombinant human IL-10R alpha/IL10RA protein
IL-10 R alpha, also known as Interleukin-10 receptor subunit alpha, is structurally related to interferon receptors. It has been shown to mediate the immunosuppressive signal of interleukin 10, and thus inhibits the synthesis of proinflammatory cytokines. This receptor is reported to promote survival of progenitor myeloid cells through the insulin receptor substrate-2/PI 3-kinase/AKT pathway. Activation of this receptor leads to tyrosine phosphorylation of JAK1 and TYK2 kinases. It has been shown to interact with Interleukin 10 and Janus kinase 1. Recombinant human IL-10 R alpha, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Human IL-10 R alpha inhibits Human IL-10 induced cell proliferation in the MC/9 mouse mast cells. The ED50 range is ≤300 ng/mL.
Product Name Alternative
Expression System
Baculovirus
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE, Bioactivity
Concentration
0.2 mg/mL (determined by Bradford assay)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 90% by SDS-PAGE
Bioactivity
Measured by its ability to inhibit proliferation using MC/9 mouse mast cells. The ED50 for this effect is ≤300 ng/mL with Human IL-10.
Molecular Weight
25.2 kDa (220aa)
Additionnal Information
References & Citations
Josephson K, et al. (2001) Acta Crystallogr D Biol Crystallogr. 57 (Pt 12) :1908-11.; ; Usacheva A, etal. (2002) J Immunol. 169 (3) :1302-8.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol
Scientific Category
Hormones & Cytokines
NCBI Accession Number
NP_001549
Uniprot Accession Number
Q13651
Species
Human
N Terminal Sequence
His22
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