Recombinant human Neprilysin/CD10 protein

Neprilysin, also known as MME, is a zinc metallopeptidase expressed at the cell surface of a variety of cells. It degrades the amyloid beta peptide whose abnormal misfolding and aggregation in neural tissue has been implicated as a cause of Alzheimer's disease. It is expressed in a wide variety of tissues and is particularly abundant in kidney. It is also a common acute lymphocytic leukemia antigen that is an important cell surface marker in the diagnosis of human acute lymphocytic leukemia (ALL) . It is of use in hematological diagnosis since it is expressed by early B, pro-B and pre-B lymphocytes, and by lymph node germinal centers. Recombinant human neprilysin, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is > 5,000 pmol/min/ug in which one unit will convert 1.0 pmole of Mca-SEVNLDAEFRK (Dnp) RR-NH2 to MCA- Pro-Leu-OH per minute at pH 8.8 at 25C.

Product Name Alternative

MME, Neprilysin, CALLA, CD10, CMT2T, NEP, SCA43, SFE, Atriopeptidase, Common acute lymphocyticleukemia antigen, Enkephalinase, Neutral endopeptidase, Skin fibroblast elastase, Membrane metalloendopeptidase, EPN

Expression System

Baculovirus

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

1 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Bioactivity

Specific activity is > 5,000pmol/min/ug in which one unit will convert 1.0pmole of Mca-SEVNLDAEFRK (Dnp) RR-NH2 to MCA- Pro-Leu-OH per minute at pH 8.8 at 25C.

Molecular Weight

80.9 kDa (708aa)

Additionnal Information

Neprilysin, ATGP3907-10 µg, ATGP3907-20 µg, ATGP3907-50 µg, ATGP3907-100 µg, ATGP3907-250 µg, ATGP3907-500 µg, ATGP3907-1 mg, ATGP3907-10, ATGP3907-20, ATGP3907-50, ATGP3907-100, ATGP3907-250, ATGP3907-500, ATGP3907-1

References & Citations

Auer-Grumbach M., et al. (2016) Am J Hum Genet. 99:607-623.; ; Hama E., et al. (2005) Med Hypotheses. 65:498-500.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 100 mM NaCl, 0.1mM PMSF, 10% glycerol

Scientific Category

Immunology

NCBI Accession Number

NP_000893

Uniprot Accession Number

P08473

Species

Human

N Terminal Sequence

Ala

AA Sequence

YDDGICK SSDCIKSAAR LIQNMDATTE PCTDFFKYAC GGWLKRNVIPETSSRYGNFDILRDELEVVL KDVLQEPKTE DIVAVQKAKA LYRSCINESA IDSRGGEPLLKLLPDIYGWPVATENWEQKY GASWTAEKAI AQLNSKYGKK VLINLFVGTD DKNSVNHVIHIDQPRLGLPSRDYYECTGIY KEACTAYVDF MISVARLIRQ EERLPIDENQ LALEMNKVMELEKEIANATAKPEDRNDPML LYNKMTLAQI QNNFSLEING KPFSWLNFTN EIMSTVNISITNEEDVVVYAPEYLTKLKPI LTKYSARDLQ NLMSWRFIMD LVSSLSRTYK ESRNAFRKALYGTTSETATWRRCANYVNGN MENAVGRLYV EAAFAGESKH VVEDLIAQIR EVFIQTLDDLTWMDAETKKRAEEKALAIKE RIGYPDDIVS NDNKLNNEYL ELNYKEDEYF ENIIQNLKFSQSKQLKKLREKVDKDEWISG AAVVNAFYSS GRNQIVFPAG ILQPPFFSAQ QSNSLNYGGIGMVIGHEITHGFDDNGRNFN KDGDLVDWWT QQSASNFKEQ SQCMVYQYGN FSWDLAGGQHLNGINTLGENIADNGGLGQA YRAYQNYIKK NGEEKLLPGL DLNHKQLFFL NFAQVWCGTYRPEYAVNSIKTDVHSPGNFR IIGTLQNSAE FSEAFHCRKN SYMNPEKKCR VW