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Recombinant mouse Carboxyl Ester Lipase/CEL protein

CEL, also known as bile salt-activated lipase, is one of the Type-B carboxyl esterase/lipase family of enzymes. It is an enzyme produced by the adult pancreas and aids in the digestion of fats. Also, it catalyzes fat and vitamin absorption and acts in concert with pancreatic lipase and colipase for the complete digestion of dietary triglycerides. This protein is highly expressed by pancreatic acinar cells and secreted into the gastrointestinal tract. Recombinant mouse CEL, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is > 100,000 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0 umoleof p-nitrophenyl butyrate to p-nitrophenol per minute at pH 7.5 at 25C

Product Name Alternative

Cel, 1810036E18Rik, BAL, BSSL, Carboxyl ester lipase, Cholesterol esterase, Pancreaticlysophospholipase, Sterol esterase, Lip1, Bile salt-activated lipase, Bile salt-stimulated lipase

Expression System

Baculovirus

Antigen Species

Mouse

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

0.5 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Bioactivity

Specific activity is > 100,000pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0 umole of p-nitrophenyl butyrate to p-nitrophenol per minute at pH 7.5 at 25C

Molecular Weight

64.5 kDa (585aa)

Additionnal Information

Carboxyl Ester Lipase, CEL, ATGP3905-10 µg, ATGP3905-20 µg, ATGP3905-50 µg, ATGP3905-100 µg, ATGP3905-250 µg, ATGP3905-500 µg, ATGP3905-1 mg, ATGP3905-10, ATGP3905-20, ATGP3905-50, ATGP3905-100, ATGP3905-250, ATGP3905-500, ATGP3905-1

References & Citations

Xiao X., et al. (2016) J Biol Chem. 291:23224-23236.; ; Shindo K., et al. (2017) Oncotarget. 8:50824-50831.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Cell Cycle

NCBI Accession Number

NP_034015

Uniprot Accession Number

Q64285

Species

Mouse

N Terminal Sequence

Ala21

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