Recombinant mouse M-CSF protein

M-CSF, also known as macrophage colony-stimulating factor 1 isoform 1, is one of the hematopoietic growth factors that regulate the growth and differentiation of blood cells. This protein is produced by monocytes, granulocytes, endothelial cells, and fibroblasts. It stimulates the formation of macrophage colonies, enhancesantibody-dependent, cell-mediated cytotoxicity by monocytes and macrophages, and inhibits bone resorption byosteoclasts. It plays an essential role in the regulation of survival, proliferation and differentiation ofhematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. This proteinpromotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and ininflammatory processes. Recombinant mouse M-CSF, fused to His-tag at C-terminus, was expressed in insect celland purified by using conventional chromatography techniques.