Recombinant human SPARC protein

SPARC, also known as osteonectin, is a member of secreted matricellular proteins. It is produced by fibroblasts, capillary endothelial cells, platelets and macrophages, especially in areas of tissue morphogenesis and remodeling. This protein regulates cell growth through interactions with the extracellular matrix and cytokines and binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. It functions as modulating cell-cell and cell-matrix interactions and its de-adhesive and growth inhibitory properties in non-transformed cell. It is secreted by osteoblasts during bone formation, initiating mineralization and promoting mineral crystal formation. A correlation between SPARC over-expression and ampullary cancers and chronic pancreatitis has been found. It also shows affinity for collagen in addition to bone mineral calcium. Recent studies have also demonstrated a role for SPARC in sensitizing therapy-resistant cancers. Notably, it is linked to human obesity. Recombinant human SPARC, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

SPARC, Basement-membrane protein 40, BM-40, Osteonectin, ON, Secreted protein acidic and rich in cysteine, OI17

Expression System

Baculovirus

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Molecular Weight

33.8 kDa (295aa)

Additionnal Information

SPARC, ATGP3902-10 µg, ATGP3902-20 µg, ATGP3902-50 µg, ATGP3902-100 µg, ATGP3902-250 µg, ATGP3902-500 µg, ATGP3902-1 mg, ATGP3902-10, ATGP3902-20, ATGP3902-50, ATGP3902-100, ATGP3902-250, ATGP3902-500, ATGP3902-1

References & Citations

McDonald LT., et al. (2018) Am J Physiol Heart Circ Physiol. 315 (1) :H92-H100.; ; Wang B., et al. (2018) J Biol Regul Homeost Agents. 32:1525-1531.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Cell Cycle

NCBI Accession Number

NP_003109

Uniprot Accession Number

P09486

Species

Human

N Terminal Sequence

Ala

AA Sequence

APQQEAL PDETEVVEET VAEVTEVSVG ANPVQVEVGE FDDGAEETEE EVVAENPCQN HHCKHGKVCE LDENNTPMCV CQDPTSCPAP IGEFEKVCSN DNKTFDSSCH FFATKCTLEG TKKGHKLHLD YIGPCKYIPP CLDSELTEFP LRMRDWLKNV LVTLYERDED NNLLTEKQKL RVKKIHENEK RLEAGDHPVE LLARDFEKNY NMYIFPVHWQ FGQLDQHPID GYLSHTELAP LRAPLIPMEH CTTRFFETCD LDNDKYIALD EWAGCFGIKQ KDIDKDLVI