Recombinant human CD8 alpha protein

CD8 alpha, also known as T-cell surface glycoprotein CD8 alpha chain isoform 1, is a member of integralmembrane glycoprotein that plays an essential role in the immune response and serves multiple functions inresponses. It interacts with the T-cell receptor and the MHC class I proteins presented by antigen presentingcells. Homodimers of this protein at the cell surface provides a survival mechanism allowing conjugation and lysisof multiple target cells in NK-cells. Recombinant human CD8 alpha, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

CD8A, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2, CD8a, MAL, CD8, Leu2, p32, T-cell surface glycoprotein CD8 alpha chain isoform 1

Expression System

Baculovirus

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Molecular Weight

18.7 kDa (170aa)

Additionnal Information

CD8 a, ATGP3901-10 µg, ATGP3901-20 µg, ATGP3901-50 µg, ATGP3901-100 µg, ATGP3901-250 µg, ATGP3901-500 µg, ATGP3901-1 mg, ATGP3901-10, ATGP3901-20, ATGP3901-50, ATGP3901-100, ATGP3901-250, ATGP3901-500, ATGP3901-1

References & Citations

Harland KL., et al. (2014) Nat Commun. 5:3547.; ; Chen S., et al. (2019) J Cancer. 10:697-707.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Immunology

NCBI Accession Number

NP_001759

Uniprot Accession Number

P01732

Species

Human

N Terminal Sequence

Ala

AA Sequence

SQFRVSP LDRTWNLGET VELKCQVLLS NPTSGCSWLF QPRGAAASPT FLLYLSQNKP KAAEGLDTQR FSGKRLGDTF VLTLSDFRRE NEGYYFCSAL SNSIMYFSHF VPVFLPAKPT TTPAPRPPTP APTIASQPLS LRPEACRPAA GGAVHTRGLD FACD